What color are non acid fast organisms after staining?

Answer 1

Acid-fast organisms are characterized by wax-like cell walls.Because the cell wall is so resistant to most compounds, acid-fast organisms require a special staining technique.
1. The primary stain used in acid-fast staining, carbolfuchsin, is lipid-soluble and contains phenol, which helps the stain penetrate the cell wall. (violet or purple).
2. This is further assisted by the addition of heat.
3. The smear is then rinsed with a very strong decolorizer, which strips the stain from all non-acid-fast cells but does not permeate the cell wall of acid-fast organisms.
4. The decolorized non-acid-fast cells then take up the counterstain. (safranin- red).
Gram positive bacteria will stain purple.
Gram negative bacteria will stain red/pink.

Answer 2

Non-acid-fast organisms are typically stained blue after staining with a primary stain, such as crystal violet, in the acidic stain technique. This is because the primary stain can penetrate the cell walls of non-acid-fast organisms and dye them blue.