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The function of a counterstain in acid-fast stains is to dye the non acid-fast bacteria cells a different color than the acid-fast cells. With two different colors present on a slide, the contrast between the two types (acid-fast and non acid-fast) is more distinct. The more distinct view of cells will assist in observations of a slide.
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Why is it essential that the primary stain and the counterstain be of contrasting colors?
It is essential that primary stain and the counterstain be of contrasting colors so that the target of the primary stain can easily be differentiated on a contrasting background.
Which step of a gram stain can be omitted without affecting determination of the reaction?
you can omit the last step - the safranin counterstain
What is the counterstain in a spore stain?
the counter stain is safranin 0.5%
Assume you stain Bacillus by applying malachite green with heat and then counterstaining with safranin Through the microscope the green structures are?
The malachite green penetrates the endospores, the counterstain of safranin will stain the vegetative cells. Endospores - greenish-blue Vegetative Cells - Red/Pink
What is the function of the bundle of HIS?
What function of the bundle of his
Which is the Counterstain used in PAS staining?
The counterstain for PAS is hematoxylin which stains nucleic acids blue.
What is the meaning of stain for?
meaning of counterstain
Could other dyes be used as a counterstain?
no
What dye is used in counterstain?
i wouldn't have asked you if i know it
Why is it essential that the primary stain and the counterstain be of contrasting colors?
It is essential that primary stain and the counterstain be of contrasting colors so that the target of the primary stain can easily be differentiated on a contrasting background.
What happen if you forget to add crystal violet to a stain?
You don't get a counterstain.
Which step of a gram stain can be omitted without affecting determination of the reaction?
you can omit the last step - the safranin counterstain
What is the application of heat not required during the ap plication of the counterstain in acid fast stain?
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What is the counterstain in a spore stain?
the counter stain is safranin 0.5%
What would happen if you forgot to counterstain with safranin?
Gram neg cells would remain clear
Is serratia marcescens acid-fast?
Serratia marcesens is an acid fast negative bacillus. It appears blue under the microscope.
Why does the counterstain not change the color of all of the cells is it because the primary stain repels or masks the secondary stain?
In a gram stain the primary stain is crystal violet. Iodine then sets that dye into the gram positive cells while alcohol washes out the crystal violet from the gram negative cells. Then safranin, which is the counterstain in a gram stain, is used to dye the rest of the bacteria. This is the example I can give you of why a counterstain does not change the look in all the cells. Though safranin stains all the cells, the gram positive cells that were dyed purple from crystal violet don't look pink - only the gram negative do.
