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safranin
Emmanuel Ward
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Why is the counter stain for gram stain procedure differ from the counter stain used for acid fast procedure?
Safranin
What type of dye is used to stain the specimen when the acid-fast stain and the gram stain are used?
Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
Why is methylene blue is an appropriate dye for staining bacterial cells?
It is used in gram staining to differentiate gram negative and gram positive bacteria. After being dyed, the cells are washed with ethanol. Gram positive bacteria will retain the methylene blue due to the amount of peptidoglycan in their cell walls, where gram negative cells will not. Iodine is used as a counter stain, which is up-taken by gram negative cells. After the gram staining procedure is finished, gram positive cells will appear dark purple or blue due to the retained methylene blue. Gram negative cells will appear pink or red due to the iodine counter stain.
What bacterial staining technique is it when a basic dye is utilized to stain bacterial cells?
A basic dye used in gram staining is crystal violet.
Describe the microscopic appearance of encapsulated streptococcus if stained with safranin?
Safranin (red) is used in gram staining and endospore staining as the secondary stain. Nigrosin is used in negative staining, staining only the background and not the bacteria. Therefore, the bacteria within the capsule would stain red from the safranin. (Like in endospore staining and negative gram staining, safranin would stain the bacteria red.) Nigrosin would stain the background of the organism just as it would in negative staining. Bacteria (within capsul): stained safranin red Capsule (outer layer of bacteria): clear Background of organism: stained dark with Nigrosin
Why is the counter stain for gram stain procedure differ from the counter stain used for acid fast procedure?
Safranin
What is gram positive strain?
Gram-positive bacterium are those that are stained dark blue or violet by Gram Staining. This is in contrast to Gram-Negative Bacterium, which cannot retain the crystal violet stain, instead taking up the counter-stain and appearing red or pink. Gram-positive organisms are able to retain the crystal violet stain because of the high amount of peptidoglycan in the cell wall. Gram-positive cell walls typically lack the outer membrane found in Gram-negative bacteria.
Why gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Why is Gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Is carbol fuchsin the same as safranin?
No. safranin is the classic stain used in gram staining. Concentrated Carbol Fushin is mainly used for the ZN staining procedure to stain organisms such as Vibrio cholerae and Cryptosporidium. Diluted Carbol Fushin can however be used as a replacement counterstain for Safranin in the gram stain.
What type of dye is used to stain the specimen when the acid-fast stain and the gram stain are used?
Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
Why is methylene blue is an appropriate dye for staining bacterial cells?
It is used in gram staining to differentiate gram negative and gram positive bacteria. After being dyed, the cells are washed with ethanol. Gram positive bacteria will retain the methylene blue due to the amount of peptidoglycan in their cell walls, where gram negative cells will not. Iodine is used as a counter stain, which is up-taken by gram negative cells. After the gram staining procedure is finished, gram positive cells will appear dark purple or blue due to the retained methylene blue. Gram negative cells will appear pink or red due to the iodine counter stain.
What bacterial staining technique is it when a basic dye is utilized to stain bacterial cells?
A basic dye used in gram staining is crystal violet.
What is the stain most useful in clinicians to decide for bacterial infection?
Methylene blue a basic stain is generally used to identify the external morphology of bacteria.The other stain which is used as differential stain and which can also differentiate the baceteia on the basis of their cell wall is gram stain i.e. Crystal voilet and is counter stained with Saffranine
What are the different techniques used in Biology?
Gram staining: This is to determine if a bacterial cell is Gram positive or negative. This uses Crystal violet dye, Gram's iodine as a mordant, Ethyl Alcohol as a decolorization medium, and Safranin as a secondary dye. Spore staining: Primary dye is Malachite green, then slide is placed over boiling beaker, cooled, rinsed with water, then Safranin is used as a counter stain. This test is used to show whether a bacteria is a spore former. Acid fast staining: Primary dye is Carbolfuchsin, heated over beaker like the spore stain, acid alcohol is used as the decolorizing agent, and Methylyene Blue is used as a counter stain. This is used to show bacteria with acid-fast walls, which have a thick waxy lipid around them. These are the most commonly used staining techniques with Bacteria.
What is the name of the paper used to dry slides after gram staining?
Gram staining is used to identify whether a bacterium is gram positive or gram negative. Slides can be dried using filter paper or tissues. The technique is based on the reaction of stain that happens with the membrane of bacteria.
Describe the microscopic appearance of encapsulated streptococcus if stained with safranin?
Safranin (red) is used in gram staining and endospore staining as the secondary stain. Nigrosin is used in negative staining, staining only the background and not the bacteria. Therefore, the bacteria within the capsule would stain red from the safranin. (Like in endospore staining and negative gram staining, safranin would stain the bacteria red.) Nigrosin would stain the background of the organism just as it would in negative staining. Bacteria (within capsul): stained safranin red Capsule (outer layer of bacteria): clear Background of organism: stained dark with Nigrosin
