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What color would the G- cell be if not counter stained with safranin?
The color of the G- cell would be transparent or colorless if not counterstained with safranin. Safranin is a red/pink dye used in the Gram staining process to distinguish between Gram-positive and Gram-negative bacteria, so without this counterstain, the G- cell would not have a visible color.
If one stained a gram-positive cell only with safranin?
If a gram-positive cell is stained only with safranin, it would likely appear pink or red under a microscope. This is because safranin is a counterstain used in the Gram staining procedure to colorize gram-negative bacteria, whereas gram-positive bacteria retain the crystal violet primary stain and appear purple.
What would be the appearance of the gram positive bacterium if you forget to counterstain with safranin?
If you forget to counter stain color of Gram positive would be violet or blue . The above answer is good. Here is why the above answer is good. Yes it would still be Violet or blue. Gram positive bacteria are gram positive, because it holds onto the crystal violet stain that washes out of gram negative bacteria. Counterstaining with safranian turns gram negative bacteria pink to red only because the crystal violet has washed out of the gram negative. The lighter safranian has little to no effect on gram positive bacteria. The cause of the difference has to do with the makeup of the cell wall in the different bacteria.
If Congo red is used instead of safranin in the gram stain technique what would happen?
Using Congo red instead of safranin in the Gram stain technique would not provide accurate results. Safranin is essential for counterstaining gram-negative bacteria, whereas Congo red would not differentiate between gram-positive and gram-negative cells due to its staining properties. This would lead to incorrect classification of bacteria in the Gram stain.
What happens if not enough alcohol is applied to a gram stain?
Both bacteria types would be stained by the safranin. When the iodine is added, safranin would be "set" in the positive. The decolorizer would wash out the safranin and then application of the crystal violet would stain the negative.
What color would the G- cell be if not counter stained with safranin?
The color of the G- cell would be transparent or colorless if not counterstained with safranin. Safranin is a red/pink dye used in the Gram staining process to distinguish between Gram-positive and Gram-negative bacteria, so without this counterstain, the G- cell would not have a visible color.
If one stained a gram-positive cell only with safranin?
If a gram-positive cell is stained only with safranin, it would likely appear pink or red under a microscope. This is because safranin is a counterstain used in the Gram staining procedure to colorize gram-negative bacteria, whereas gram-positive bacteria retain the crystal violet primary stain and appear purple.
What would be the appearance of the gram positive bacterium if you forget to counterstain with safranin?
If you forget to counter stain color of Gram positive would be violet or blue . The above answer is good. Here is why the above answer is good. Yes it would still be Violet or blue. Gram positive bacteria are gram positive, because it holds onto the crystal violet stain that washes out of gram negative bacteria. Counterstaining with safranian turns gram negative bacteria pink to red only because the crystal violet has washed out of the gram negative. The lighter safranian has little to no effect on gram positive bacteria. The cause of the difference has to do with the makeup of the cell wall in the different bacteria.
In spore staining what would happen it you used safarin as the primary stain and malachite green as the counterstain?
The spore would appear to be red as the safranin is heat driven into the many layers of the spore, however, as Malachite green has a weak affinity and is water soluble, it will not likely bind to the spore wall or the cell wall. You might have traces of green on the slide if any, but it will be very little. Your vegetative cells will be pink as well.
If Congo red is used instead of safranin in the gram stain technique what would happen?
Using Congo red instead of safranin in the Gram stain technique would not provide accurate results. Safranin is essential for counterstaining gram-negative bacteria, whereas Congo red would not differentiate between gram-positive and gram-negative cells due to its staining properties. This would lead to incorrect classification of bacteria in the Gram stain.
What would happen if you ate a breath mint and forgot to chew it?
you would have a minty choke!
If mordant not applied how will e.coli appear?
Without mordant, E. coli would appear pink or red after Gram staining due to retaining the safranin counterstain, indicating that it is a Gram-negative bacterium. The absence of the mordant would prevent the crystal violet stain from binding strongly to the peptidoglycan layer in the cell wall, leading to this coloration.
What would happen to your mail if you forgot to put a stamp on it?
The post office would return it to the return address.
What happens if not enough alcohol is applied to a gram stain?
Both bacteria types would be stained by the safranin. When the iodine is added, safranin would be "set" in the positive. The decolorizer would wash out the safranin and then application of the crystal violet would stain the negative.
Describe the microscopic appearance of encapsulated streptococcus if stained with safranin?
Safranin (red) is used in gram staining and endospore staining as the secondary stain. Nigrosin is used in negative staining, staining only the background and not the bacteria. Therefore, the bacteria within the capsule would stain red from the safranin. (Like in endospore staining and negative gram staining, safranin would stain the bacteria red.) Nigrosin would stain the background of the organism just as it would in negative staining. Bacteria (within capsul): stained safranin red Capsule (outer layer of bacteria): clear Background of organism: stained dark with Nigrosin
What would happen if someone forgot to add the starch to the beverage before they began adding iodine?
you die
What would happen If steel makers forgot to add enough chromium and nickel to a batch of steel?
Then the finished product would not be stainless steel.
