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If a gram-positive cell is stained only with safranin, it would likely appear pink or red under a microscope. This is because safranin is a counterstain used in the Gram staining procedure to colorize gram-negative bacteria, whereas gram-positive bacteria retain the crystal violet primary stain and appear purple.
What else can I help you with?
What cell structures can be seen in a stain onion cell?
The structure that is seen is the cell wall. This keeps the shape of the cell and is only found in plant cells. The organelles that can be see in a stained onion cells all depends on your microscope. Under a x400 light microscope we could see the cell wall, cell membrane, nucleus and cytoplasm,
What happens if you use gram stain on a virus?
Most likely nothing. The gram stain will only stain the peptidoglycan cell wall of bacteria. Most viruses have a protein coat called a capsid. The capsid will not take up either the crystal violet or the safranin of a Gram stain.
Use of trypan blue in cell counting?
u count dead cells stained by trypan blue. trypan blue enters the cells only when it has died since when a cell dies it looses its property of controlling selective inlet of micromolecules. When cells r alive it doesn't allow inlet of trypan blue so viable cells r not stained. viable cells = total cells - trypan blues stained cells Nitin Ghoshal Dept of Biotechnology NEHU, Shillong, Meghalaya, India
How does thickness of peptidoglycan affect gram stain outcome?
For Gram Stain experiment. The gram-positive, g+, which stained purple in color (use Crystal violet) as the bacteria contain a thicker layer of peptidoglycan with no lipopolysaccharide and protein bound the outer membrane of the bacteria (bacteria A). The gram-negative, g-, which stained in red (if the safranin (red dye/counter stain is use) appear due to the bacteria (bacteria B) which lack of peptidoglycan and it is bound by a lipopolysaccharide and protein layer. The thicker the peptidoglycan, the more coloring agent (crystal violet ions, CV+) it can absorb. Hence, give more purple in color to the bacteria and this will be the gram-positive, g+ bacteria. On the other hands, bacteria B seem has thinner peptidoglycan thus it absorb less purple color from the CV+, and after decolourise (using alcohol) and stained with safranin (red dye), only the bacteria with lack of peptidoglycan change to red hence we can say bacteria B as gram-negative, g-. Hence the outcome is the bacteria with thicker peptidoglycan layer be the gram-positive, g+(purple color) while the thinner peptidoglycan layer be the gram-negative, g-. (red color)
Does an annimal cell have a cell wall?
No, insect cell has not a cell wall, insect cell has cell membrane. Only plant cell has cell has cell wall.
Describe the microscopic appearance of encapsulated streptococcus if stained with safranin?
Safranin (red) is used in gram staining and endospore staining as the secondary stain. Nigrosin is used in negative staining, staining only the background and not the bacteria. Therefore, the bacteria within the capsule would stain red from the safranin. (Like in endospore staining and negative gram staining, safranin would stain the bacteria red.) Nigrosin would stain the background of the organism just as it would in negative staining. Bacteria (within capsul): stained safranin red Capsule (outer layer of bacteria): clear Background of organism: stained dark with Nigrosin
What cell structures can be seen in a stain onion cell?
The structure that is seen is the cell wall. This keeps the shape of the cell and is only found in plant cells. The organelles that can be see in a stained onion cells all depends on your microscope. Under a x400 light microscope we could see the cell wall, cell membrane, nucleus and cytoplasm,
What happens if you use gram stain on a virus?
Most likely nothing. The gram stain will only stain the peptidoglycan cell wall of bacteria. Most viruses have a protein coat called a capsid. The capsid will not take up either the crystal violet or the safranin of a Gram stain.
Are chromosomes visible when stained?
Chromosomes can be seen using a light microscope (LM). Mitotic cells can be easily visualised. The study of chromosomes using LM is termed Karyology, the chromosomes are prepared by a variety of techniques and analyses can be performed.
What is gram stain of candida?
Candida is a fungi and cannot be gram stained. Only bacterias can be gram stained as either positive or negative.
What are two organelles that shoukd be visible in a cheek cell leb but arent due to the lighting?
They only thing you can see in a cheek cell would be the nucleus and the cellular membrane. These cells must be stained because they won't be visible under a light microscope all by themselves.
Use of trypan blue in cell counting?
u count dead cells stained by trypan blue. trypan blue enters the cells only when it has died since when a cell dies it looses its property of controlling selective inlet of micromolecules. When cells r alive it doesn't allow inlet of trypan blue so viable cells r not stained. viable cells = total cells - trypan blues stained cells Nitin Ghoshal Dept of Biotechnology NEHU, Shillong, Meghalaya, India
What is the current theory about the mechanism of the gram stain?
The gram stain is a basic differential stain used to determine if a bacterial cell is gram positive or negative. Gram positive cells have a thick peptidoglycan layer that will trap the crystal violet iodine crystalls and apear purple. Gram negative cells only have a thin peptidoglycan layer that allows the crystals to diffuse out of the cell and will only be seen with the application of a counterstain, such as safranin which turns the cells pink.
What color is the cytoplasm in plant cells?
The Natural Color Appeared In The Plant Cell is Green And The Structures That Give The Plant Cell Is The Chloroplasts.
What color is a chromosome in a Plant Cell?
A chromosome in a plant cell is typically green in color. This is due to the presence of chlorophyll, which is a green pigment that helps the plant cell to absorb light for photosynthesis.
Which parts of the onion cells stood out the most after they were stained?
Only you know that because you did the experiment not us.
What is differences do you see in the stained and unstained onion cells?
Oh, dude, when you stain an onion cell, it's like giving it a little makeover - you can actually see the details more clearly under a microscope because the stain highlights different structures. Unstained cells are just chilling in their natural state, not trying to impress anyone with their flashy colors. So yeah, staining basically just helps you spot the cool stuff inside the cell easier.
