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For Gram Stain experiment.
The gram-positive, g+, which stained purple in color (use Crystal violet) as the bacteria contain a thicker layer of peptidoglycan with no lipopolysaccharide and protein bound the outer membrane of the bacteria (bacteria A).
The gram-negative, g-, which stained in red (if the safranin (red dye/counter stain is use) appear due to the bacteria (bacteria B) which lack of peptidoglycan and it is bound by a lipopolysaccharide and protein layer.
The thicker the peptidoglycan, the more coloring agent (crystal violet ions, CV+) it can absorb. Hence, give more purple in color to the bacteria and this will be the gram-positive, g+ bacteria. On the other hands, bacteria B seem has thinner peptidoglycan thus it absorb less purple color from the CV+, and after decolourise (using alcohol) and stained with safranin (red dye), only the bacteria with lack of peptidoglycan change to red hence we can say bacteria B as gram-negative, g-.
Hence the outcome is the bacteria with thicker peptidoglycan layer be the gram-positive, g+(purple color) while the thinner peptidoglycan layer be the gram-negative, g-. (red color)
What else can I help you with?
Do gram positive stain pink?
No, gram-positive bacteria do not stain pink; they stain purple during the Gram staining procedure. This occurs because their thick peptidoglycan cell wall retains the crystal violet dye used in the staining process. In contrast, gram-negative bacteria, which have a thinner peptidoglycan layer and an outer membrane, stain pink due to the counterstain (safranin) used after the crystal violet is washed out.
What is the gram stain for clostridium perfringens?
Clostridium perfringens is a Gram-positive bacterium, meaning it will stain purple/blue with the Gram stain procedure due to its thick peptidoglycan layer.
Why is peptidoglycan a major structural protein divide bacterial into two major groups?
Peptidoglycan is a crucial structural component of bacterial cell walls, providing rigidity and shape. Its composition and thickness differ between two major groups of bacteria: Gram-positive bacteria have a thick peptidoglycan layer that retains the crystal violet stain, while Gram-negative bacteria possess a thinner layer sandwiched between an outer membrane and the cytoplasmic membrane, leading to a pink coloration after Gram staining. This fundamental difference in peptidoglycan structure plays a key role in the classification and identification of bacteria.
Why would bacteria stain gram variable?
A gram-negative cell will lose its outer membrane and the peptidoglycan layer is left exposed. or it is best to use younger cells ( 12-24hr) because older gram positive bacteria are subject to break down of the cell wall by enzymes that are produced with age which may result ingram variable staining.
What is the difference between gram plus and?
Gram positive bacteria stain purple with the Gram stain. This is because of the fact that they contain a thick layer of murein in their cell wall, which takes up the stain very well. Gram negative bacteria, however, do not display the thick layer of peptidoglycan on their outer surface. Therefore, they stain red with the counter stain.
How does thickness of peptidoglycan affect the outcome of gram stain?
For Gram Stain experiment. The gram-positive, g+, which stained purple in color (use Crystal violet) as the bacteria contain a thicker layer of peptidoglycan with no lipopolysaccharide and protein bound the outer membrane of the bacteria (bacteria A). The gram-negative, g-, which stained in red (if the safranin (red dye/counter stain is use) appear due to the bacteria (bacteria B) which lack of peptidoglycan and it is bound by a lipopolysaccharide and protein layer. The thicker the peptidoglycan, the more coloring agent (crystal violet ions, CV+) it can absorb. Hence, give more purple in color to the bacteria and this will be the gram-positive, g+ bacteria. On the other hands, bacteria B seem has thinner peptidoglycan thus it absorb less purple color from the CV+, and after decolourise (using alcohol) and stained with safranin (red dye), only the bacteria with lack of peptidoglycan change to red hence we can say bacteria B as gram-negative, g-. Hence the outcome is the bacteria with thicker peptidoglycan layer be the gram-positive, g+(purple color) while the thinner peptidoglycan layer be the gram-negative, g-. (red color)
Which stain sticks to the peptidoglycan in the cell wall of bacteria?
The stain that sticks to the peptidoglycan in the cell wall of bacteria is called crystal violet.
How do you get gram positive bacilli?
You do not " get " them, but gram positive bacteria are gram positive because their cell wall is of sufficient thickness and properly constructed to hold the Gram stain. They have one thick wall of peptidoglycan that holds the stain against being washed away. Gram negative bacteria have two thinner walls which allow the stain to be washed away, basically.
Can you dilute wood stain with water to achieve a lighter shade?
No, you cannot dilute wood stain with water to achieve a lighter shade. Wood stain is designed to be used as is and diluting it with water can affect its effectiveness and the final color outcome.
What does pre-stain do and how does it affect the final outcome of the wood staining process?
Pre-stain is a wood conditioner that helps to even out the absorption of stain on the wood surface. It penetrates the wood to create a more uniform base for the stain, resulting in a smoother and more consistent finish.
Bacteria are divided into two groups based on how their cell walls absorb?
Bacteria are divided into two groups based on how their cell walls absorb the Gram stain. Gram-positive bacteria retain the stain due to their thick peptidoglycan cell wall, while Gram-negative bacteria do not retain the stain because of their thin peptidoglycan cell wall and an outer membrane.
Do gram positive stain pink?
No, gram-positive bacteria do not stain pink; they stain purple during the Gram staining procedure. This occurs because their thick peptidoglycan cell wall retains the crystal violet dye used in the staining process. In contrast, gram-negative bacteria, which have a thinner peptidoglycan layer and an outer membrane, stain pink due to the counterstain (safranin) used after the crystal violet is washed out.
What is the gram stain for clostridium perfringens?
Clostridium perfringens is a Gram-positive bacterium, meaning it will stain purple/blue with the Gram stain procedure due to its thick peptidoglycan layer.
How do you calculate mild steel thickness from stain steel thickness?
By thick control.
Why is peptidoglycan a major structural protein divide bacterial into two major groups?
Peptidoglycan is a crucial structural component of bacterial cell walls, providing rigidity and shape. Its composition and thickness differ between two major groups of bacteria: Gram-positive bacteria have a thick peptidoglycan layer that retains the crystal violet stain, while Gram-negative bacteria possess a thinner layer sandwiched between an outer membrane and the cytoplasmic membrane, leading to a pink coloration after Gram staining. This fundamental difference in peptidoglycan structure plays a key role in the classification and identification of bacteria.
Gram-positive bacteria have a thick cell wall composed of?
Gram positive bacteria are so called because their cell walls are one thick layer of peptidoglycan, basically, so the stain is trapped in the cell wall and not rinsed away.
What is pre-stain and how does it affect the final outcome of a wood staining project?
Pre-stain is a wood conditioner that is applied before staining to help the wood absorb the stain more evenly. It helps prevent blotchiness and uneven coloration, resulting in a smoother and more consistent finish on the wood.
