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Gram positive bacteria stain purple with the Gram stain. This is because of the fact that they contain a thick layer of murein in their cell wall, which takes up the stain very well. Gram negative bacteria, however, do not display the thick layer of peptidoglycan on their outer surface. Therefore, they stain red with the counter stain.

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What cell structure is significantly different between gram-positive and gram-negative bacteria?

The difference between a gram positive and gram negative bacteria is the thickness/presence of the peptidoglycan layer secreted on the outside of the plasma membrane


Can you tell an organisms gram stain by simple staining?

Gram stain is not a simple stain because simple stains do not use two or more stains. Gram stain is a differential stain differentiating between Gram positive (blue-black) and Gram negative (pink-red).


Difference between Gram atom and gram molecule?

It's much simpler than you're probably thinking. You use the term gram atomic weight for atoms, and the term gram molecular weight for molecules. That's all. The gram molecular weight of a molecule is the sum of the gram atomic weights of the atoms making up that molecule.


What color is a gram positive cell and what color is a gram negative cell?

In a Gram stain procedure, gram-positive cells appear purple due to their thick peptidoglycan layer, which retains the crystal violet stain. In contrast, gram-negative cells appear pink or red, as their thinner peptidoglycan layer does not retain the crystal violet and is counterstained with safranin. This difference in color is key to differentiating between the two types of bacteria.


What is the difference between positive gram and negative gram?

The major difference between gram positive and the gram negative bacteria is the structure of their cell wall. The gram positive bacteria have two layers a plasma membrane and outer to it a peptidoglycan layer. But the gram negative bacteria have another layer other than the cell wall. While the gram negative bacteria has three layers. It has a plasma membrane that is called as the inner membrane, then a peptidoglycan layer and finally an outer membrane of phospholipids and lipopolysaccharides. There is space between the inner membrane and the peptidoglycan layer called as the periplasmic space. Peptidoclycan layer in the gram positive bacteria are much more thicker than that of the gram negative bacteria. The molecule teichoic acid is present in gram positive bacteria and the porin proteins are characteristically present in the gram negative bacteria. Gram positive bacteria usually produce exotoxins and the gram negative bacteria usually produces endotoxins. Gram positive bacteria gives Violet colour to the gram staining and the gram negative bacteria gives pink colour to the gram staining. In gram staining the bacteria is fixed on slides and then they are treated with Crystal Violet. After that they are treated with iodine and then they are decolourised. If the bacteria is gram negative then it shows Violet colour and if it is gram negative then it shows pink colour. Initially both strains show Violet colour after Crystal Violet treatment but on the treatment with iodine and decolourisation the gram negative bacteria loose colour and become pink. This is because in the gram positive bacteria the Violet crystals bind firmly to the peptidoglycan layer and the colour is not removed in later stages. In gram positive bacteria the colour does not penetrate much because of outer membrane and is lost much during decolourisation.

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What cell structure is significantly different between gram-positive and gram-negative bacteria?

The difference between a gram positive and gram negative bacteria is the thickness/presence of the peptidoglycan layer secreted on the outside of the plasma membrane


What cell structures is significantly different between Gram positive and Gram-negative bacteria?

The difference between a gram positive and gram negative bacteria is the thickness/presence of the peptidoglycan layer secreted on the outside of the plasma membrane


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What is the difference in color between gram positive and gram negative bacteria?

Gram positive bacteria appear purple or blue when stained with the Gram stain, while gram negative bacteria appear pink or red. This color difference is due to the thickness of the cell wall and the presence of an outer membrane in gram negative bacteria.