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u count dead cells stained by trypan blue. trypan blue enters the cells only when it has died since when a cell dies it looses its property of controlling selective inlet of micromolecules. When cells r alive it doesn't allow inlet of trypan blue so viable cells r not stained. viable cells = total cells - trypan blues stained cells Nitin Ghoshal Dept of Biotechnology NEHU, Shillong, Meghalaya, India
What else can I help you with?
What does the cell membrane use for energy?
The cell membrane does not use energy.
Which cell process do organisms use when they reproduce sexually?
Single-Celled Organisms use binary fission to reproduce.
What type of cell does not use cell division to replicate?
Blood
What organelle breaks down cell can compounds into small particles that the cell can use?
The mitochondria breaks down chemical bonds the cell can use for energy
What cell would you use to electroplated silver on iron?
An electrolytic cell
Why is anti-sera A blue in color?
Anti Sera a is blue because it uses a dye called trypan blue that is mixed with a height titer antibody against a antigen, anti-sera b is colored yellow because of the dye acriflavin yellow and has the same mechanics as antisera-a
How to use a hemocytometer for cell counting and analysis?
To use a hemocytometer for cell counting and analysis, first prepare a cell suspension. Place a small amount of the suspension on the hemocytometer and allow the cells to settle. Use a microscope to count the cells in the grid squares and calculate the cell concentration. Repeat the process for accuracy and analyze the data for further insights into the cell population.
How do you properly use a hemocytometer for cell counting and analysis?
To properly use a hemocytometer for cell counting and analysis, first prepare a cell suspension and load it onto the hemocytometer. Then, place the hemocytometer under a microscope and count the cells in the grid squares. Calculate the cell concentration and analyze the data to determine cell viability or other characteristics. Remember to follow proper lab safety protocols and clean the hemocytometer after each use.
Why use citric acid in crystal violet staining?
Crystal Violet staining is a technique used in cell culture laboratories to determine concentration of cells in culture liquid. As against other means of cell staining and counting (e.g. Trypan blue) the entities counted using the Crystal Violet staining are the cell nuclei instead of whole cells. This technique has the advantage that also concentrations of aggregated cells can be determined accurately. Here comes the Citric acid in. Nuclei are situated within the cell. To extract the nuclei out of the cells Citric acid is used to make the cell burst so that the Crystal Violet can stain the nuleus and thus concentration of cells can be determined using a microscope and a counting chamber.
Which beans did the Mayan merchants use for counting?
Mayan use coco beans for counting
What you use integers for?
Counting
How do you download pictures off a Motorola citrus cell phone to the computer?
Use either Blue-tooth (if the phone AND computer are blue-tooth enabled) or a USB cable.
Where can I purchase easel backs?
Yes a cell phone and a blue tooth can be used for mobile marketing. Many businesses use the blue tooth as a hot spot for mobile marketing. You can use your cell phone outside of SMS in such ways as advertising on web pages, QR codes, and mobile applications.
How did Romans use numbers in everyday life?
Telling time, counting goods, counting money.
Why cant you use methylene blue when staining onion cells?
Methylene blue is not suitable for staining onion cells because it does not effectively bind to the distinct cell structures present in onion cells, such as the cell walls and nuclei. Toluidine blue or safranin O are commonly used stains for onion cells as they provide better contrast and visibility of cell structures.
What Tubes do you use for Basic Metabolic Panel?
red top for biochemistry tests such as lipid profile, kft, lft. purple top for cell counting grey top for blood glucose.
How do actors use math?
Counting their money?
