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u count dead cells stained by trypan blue. trypan blue enters the cells only when it has died since when a cell dies it looses its property of controlling selective inlet of micromolecules. When cells r alive it doesn't allow inlet of trypan blue so viable cells r not stained. viable cells = total cells - trypan blues stained cells Nitin Ghoshal Dept of Biotechnology NEHU, Shillong, Meghalaya, India
What else can I help you with?
What does the cell membrane use for energy?
The cell membrane does not use energy.
Which cell process do organisms use when they reproduce sexually?
Single-Celled Organisms use binary fission to reproduce.
CAN YOU USE METHYLENE BLUE USED ON A SIMPLE STAIN STAIN FOR E. COLI?
Yes, methylene blue can be used as a simple stain for E. coli. It is a basic dye that binds to the negatively charged components of bacterial cells, allowing for clear visualization under a microscope. However, it is important to note that while methylene blue provides a quick and easy method for observing cell morphology, it does not provide information on cell viability or specific characteristics of the bacteria.
What type of cell does not use cell division to replicate?
Blood
What organelle breaks down cell can compounds into small particles that the cell can use?
The mitochondria breaks down chemical bonds the cell can use for energy
Why is anti-sera A blue in color?
Anti Sera a is blue because it uses a dye called trypan blue that is mixed with a height titer antibody against a antigen, anti-sera b is colored yellow because of the dye acriflavin yellow and has the same mechanics as antisera-a
How to use a hemocytometer for cell counting and analysis?
To use a hemocytometer for cell counting and analysis, first prepare a cell suspension. Place a small amount of the suspension on the hemocytometer and allow the cells to settle. Use a microscope to count the cells in the grid squares and calculate the cell concentration. Repeat the process for accuracy and analyze the data for further insights into the cell population.
Why use citric acid in crystal violet staining?
Crystal Violet staining is a technique used in cell culture laboratories to determine concentration of cells in culture liquid. As against other means of cell staining and counting (e.g. Trypan blue) the entities counted using the Crystal Violet staining are the cell nuclei instead of whole cells. This technique has the advantage that also concentrations of aggregated cells can be determined accurately. Here comes the Citric acid in. Nuclei are situated within the cell. To extract the nuclei out of the cells Citric acid is used to make the cell burst so that the Crystal Violet can stain the nuleus and thus concentration of cells can be determined using a microscope and a counting chamber.
How do you properly use a hemocytometer for cell counting and analysis?
To properly use a hemocytometer for cell counting and analysis, first prepare a cell suspension and load it onto the hemocytometer. Then, place the hemocytometer under a microscope and count the cells in the grid squares. Calculate the cell concentration and analyze the data to determine cell viability or other characteristics. Remember to follow proper lab safety protocols and clean the hemocytometer after each use.
Which beans did the Mayan merchants use for counting?
Mayan use coco beans for counting
What is the use of neubauer chamber?
The Neubauer chamber, also known as a hemocytometer, is a specialized microscope slide used for counting and estimating the concentration of cells in a liquid sample. It features a grid pattern etched into the glass, allowing for precise counting of cells under a microscope. This tool is commonly used in laboratory settings for applications such as cell culture, blood cell counting, and determining cell viability. Its design enables accurate measurements by providing a defined volume for analysis.
What is ocular counting?
Ocular counting is a method used in various fields, particularly in biology and medicine, to estimate the number of cells or particles in a sample by visually counting them through a microscope. This technique often involves the use of a specific grid or counting chamber to facilitate accurate counting and minimize bias. Ocular counting is commonly employed in laboratories for tasks such as cell culture, hematology, and microbiology, where precise quantification is essential for research and diagnostics. However, it can be time-consuming and may have limitations in terms of accuracy compared to automated counting methods.
What you use integers for?
Counting
Where can I purchase easel backs?
Yes a cell phone and a blue tooth can be used for mobile marketing. Many businesses use the blue tooth as a hot spot for mobile marketing. You can use your cell phone outside of SMS in such ways as advertising on web pages, QR codes, and mobile applications.
Why cant you use methylene blue when staining onion cells?
Methylene blue is not suitable for staining onion cells because it does not effectively bind to the distinct cell structures present in onion cells, such as the cell walls and nuclei. Toluidine blue or safranin O are commonly used stains for onion cells as they provide better contrast and visibility of cell structures.
How did Romans use numbers in everyday life?
Telling time, counting goods, counting money.
What Tubes do you use for Basic Metabolic Panel?
red top for biochemistry tests such as lipid profile, kft, lft. purple top for cell counting grey top for blood glucose.
