Crystal Violet staining is a technique used in cell culture laboratories to determine concentration of cells in culture liquid.
As against other means of cell staining and counting (e.g. Trypan blue) the entities counted using the Crystal Violet staining are the cell nuclei instead of whole cells. This technique has the advantage that also concentrations of aggregated cells can be determined accurately.
Here comes the Citric acid in. Nuclei are situated within the cell. To extract the nuclei out of the cells Citric acid is used to make the cell burst so that the Crystal Violet can stain the nuleus and thus concentration of cells can be determined using a microscope and a counting chamber.
No, strawberries do not naturally contain citric acid. They have malic acid and citric acid.
Monohydrate citric acid has a single (=mono) molecule of water (=hydrate) attached to each molecule of citric acid, whereas anhydrous citric acid has been dried so has no water at all (no water = anhydrous).
Crystal violet is a triphenylmethane dye that can act as an acid-base indicator. In acidic solutions, it appears as a red color, while in basic solutions, it turns blue or violet. This color change is due to the different protonation states of the dye molecule at different pH levels.
Citric acid monohydrate contains one molecule of water while citric acid anhydrous does not. Citric acid monohydrate is less concentrated compared to citric acid anhydrous. The choice between the two may depend on the specific application due to differences in solubility and reactivity.
No, citric acid is not heavier than water. The density of citric acid is lower than that of water, so citric acid will float on the surface of water.
Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
citric acid is a carboxylic acid. It could form crystals if recyrstallized from suitable solvents.
Crystal Light has citric acid in it, and this may contribute to your acid reflux.
No, strawberries do not naturally contain citric acid. They have malic acid and citric acid.
Citric acid crystals are the solid form of water free citric acid, officially named: 3-carboxy-3-hydroxypentanedioic acid, C6H8O7, or citric acid monohydrate, C6H8O7.H2O
Citric acid is considered to be a weak acid.
Citric and acid ARE words, so the word form is citric acid!
The acid found in citrus fruits is called the citric acid and can provide a sour taste and helps fight of predators with its sting when it gets into your eyes so the acid in citrus fruit is citric :)
Citric acid is not malleable.
There are two acids in lemons; - #1 citric acid and #2 ascorbic acid (vitamin 'C').
Monohydrate citric acid has a single (=mono) molecule of water (=hydrate) attached to each molecule of citric acid, whereas anhydrous citric acid has been dried so has no water at all (no water = anhydrous).
Crystal violet is a triphenylmethane dye that can act as an acid-base indicator. In acidic solutions, it appears as a red color, while in basic solutions, it turns blue or violet. This color change is due to the different protonation states of the dye molecule at different pH levels.