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Methelyne BLue is a great stain to use for onion cells. It is toxic, however, and in activities that involve young kids it is often substituted.
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Why is methylene blue is an appropriate dye for staining bacterial cells?
It is used in gram staining to differentiate gram negative and gram positive bacteria. After being dyed, the cells are washed with ethanol. Gram positive bacteria will retain the methylene blue due to the amount of peptidoglycan in their cell walls, where gram negative cells will not. Iodine is used as a counter stain, which is up-taken by gram negative cells. After the gram staining procedure is finished, gram positive cells will appear dark purple or blue due to the retained methylene blue. Gram negative cells will appear pink or red due to the iodine counter stain.
Why cant methylene blue be used in place of nigrosin in negative staining?
Because negative staining requires the use of an acidic stain, which will not penetrate the cells because of the negative charge on the surface of the bacteria. As a result, the unstained cells can be easily identified against the colored background.
Why methylene blue can act on only dead yeast cells but not on live yeast cells?
Active enzymes within living cells cause methylene blue to become colorless. Since dead yeast cells have inactive/denatured enzymes, the methylene blue stays blue.
What is the standard stain for biological tissues?
Eosin and methylene blue are used for staining biolgical tissues.
Is the cell surface membrane permeable to methylene blue?
Methylene blue is used to stain animal cells, such as human cheek cells, to make their nuclei more observable. Also used to staining the blood film and used in cytology. It gives a blue coloration to the human cheek cell.
What is thee purpose of adding methylene blue to the specimen?
Adding methylene blue to a slide will stain animal cells and make the nuclei more visible.
Can food coloring be used to substitute methylene blue in staining cheek cells?
iodine
Why safranine and not methylene blue is used to stain plant cells?
Both are used in staining but for different purposes .
Staining preparation with eosin and methylene blue?
Eosin is a red stand and methylene blue is blue. The result of staining a bacterial smear with a mixture of eosin and methylene blue is that eosin is acidic and acts as a negative stain. Methylene blue is basic the smear background would turn out red while the cells would turn out blue.
What is the stain blue used to stain animal cells?
Methylene blue is used for many different staining purposes, but one of the main ones is staining RNA or DNA. In animal cells, it will stain the cytoplasm and the nucleus (the nucleus will be much darker).
What color is simple staining on a bacillus and coccus using methylene blue stain?
Methylene blue stains everything blue.
When staining ecoli with methylene blue what would you see?
blue bacilli
What is onion peel generally stained with?
methylene blue
Why is methylene blue is an appropriate dye for staining bacterial cells?
It is used in gram staining to differentiate gram negative and gram positive bacteria. After being dyed, the cells are washed with ethanol. Gram positive bacteria will retain the methylene blue due to the amount of peptidoglycan in their cell walls, where gram negative cells will not. Iodine is used as a counter stain, which is up-taken by gram negative cells. After the gram staining procedure is finished, gram positive cells will appear dark purple or blue due to the retained methylene blue. Gram negative cells will appear pink or red due to the iodine counter stain.
Why cant methylene blue be used in place of nigrosin in negative staining?
Because negative staining requires the use of an acidic stain, which will not penetrate the cells because of the negative charge on the surface of the bacteria. As a result, the unstained cells can be easily identified against the colored background.
How would the pH of the stain methylene blue affect the staining of bacteria?
the ph of the stain on the bacteria caused by methylene blue would not affect it a lot since all methylene blue is supposed to do is make it visible on the microscope for e.g.
Why methylene blue can act on only dead yeast cells but not on live yeast cells?
Active enzymes within living cells cause methylene blue to become colorless. Since dead yeast cells have inactive/denatured enzymes, the methylene blue stays blue.
