Eosin is a red stand and methylene blue is blue. The result of staining a bacterial smear with a mixture of eosin and methylene blue is that eosin is acidic and acts as a negative stain. Methylene blue is basic the smear background would turn out red while the cells would turn out blue.
It depends on what tissue you're looking at, what you want to stain, how the tissue has been stored... Besides very specific staining, there are different types of staining. For example, immunohistochemistry, which uses antibodies to stick coloured stains to cell surface receptors. Or, chemical staining - the most common is H&E staining (haemotoxylin & eosin), so if you're just having fun in a lab and want to see general structures of cells, use this one.
Adding methylene blue to a slide will stain animal cells and make the nuclei more visible.
Leishman stain is composed of a mixture of methylene blue and eosin Y dissolved in methanol. It is commonly used in laboratory settings to stain blood smears for microscopic examination, especially in the diagnosis of parasitic infections such as leishmaniasis.
Eosin methylene blue (EMB) agar is selective due to the presence of dyes eosin Y and methylene blue, which inhibit the growth of Gram-positive bacteria. This allows for the selective isolation of Gram-negative bacteria, particularly coliforms.
One substance that has a similar function as methylene blue is crystal violet. It is commonly used in staining techniques for microbiological studies and exhibits similar properties in terms of staining cells and tissues.
Bile salts in Eosin Methylene Blue agar inhibit the growth of Gram-positive bacteria, allowing for the selective growth of Gram-negative bacteria. This helps in the detection and differentiation of organisms based on their ability to ferment lactose and produce acid.
The May-Grünwald stain is a vital staining technique used primarily in hematology to visualize blood cells and their components. It relies on a combination of eosin and methylene blue, which selectively stain the cytoplasmic components and nuclei of cells, respectively. The eosin imparts a pink color to eosinophilic granules and cytoplasm, while methylene blue stains the nuclei blue, allowing for differentiation of various cell types. This staining method is particularly useful for identifying abnormalities in blood smears.
It depends on what tissue you're looking at, what you want to stain, how the tissue has been stored... Besides very specific staining, there are different types of staining. For example, immunohistochemistry, which uses antibodies to stick coloured stains to cell surface receptors. Or, chemical staining - the most common is H&E staining (haemotoxylin & eosin), so if you're just having fun in a lab and want to see general structures of cells, use this one.
Methylene blue stains everything blue.
blue bacilli
Adding methylene blue to a slide will stain animal cells and make the nuclei more visible.
Leishman stain is composed of a mixture of methylene blue and eosin Y dissolved in methanol. It is commonly used in laboratory settings to stain blood smears for microscopic examination, especially in the diagnosis of parasitic infections such as leishmaniasis.
It is a dye found in Eosin-methylene blue (EMB) agar, which permits the differentiation between enteric lactose fermenters and nonfermenters. The dye methylene blue inhibits the growth of gram positive bacteria. While the eosin acts as a pH indicator, measuring the levels of acid production due to the fermentation of lactose.Eosin is a stain that is used for staining the cells to observe the structurea nd number of cells. It is an acidophilic stain.
Eosin Methylene Blue Agar (EMB) was developed by Holt-Harris and Teague.1 This formula contains lactose and sucrose with two indicator dyes, Eosin Y and Methylene Blue. The use of Eosin Y and Methylene Blue as indicators produced sharp and distinct differentiation between colonies of lactose fermenting and nonfermenting organisms. Sucrose is included to detect coliforms that ferment sucrose more readily than lactose. EMB Agar is selective due to the presence of an inhibitor and differential based on the ability of some organisms to ferment carbohydrates with the absorption of an Eosin Y and Methylene Blue complex.
Eosin methylene Blue Agar contains peptone (a sugar of glucose), lactose, sucrose, and the dyes eosin Y and methylene blue. The sugars provide fermentable substrates to encourage growth of fecal coliforms. The dyes inhibit growth of Gram-positive organisms.
Eosin methylene blue (EMB) agar is selective due to the presence of dyes eosin Y and methylene blue, which inhibit the growth of Gram-positive bacteria. This allows for the selective isolation of Gram-negative bacteria, particularly coliforms.
Hematoxylin and eosin (H&E) stain is the most commonly used standard stain for biological tissues. Hematoxylin stains cell nuclei blue/purple, while eosin stains cytoplasm and extracellular matrix pink. This combination helps to visualize cellular structures and tissue organization under a microscope.