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What color is simple staining on a bacillus and coccus using methylene blue stain?
Wiki User
∙ 13y ago
Methylene blue stains everything blue.
Wiki User
∙ 13y ago
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What does a simple stain of bacillus megaterium look like under a microscope?
Simple or gram? Simple uses methylene blue, gram uses crystal violet
What is the advantage of the gram stain over simple stains such as methylene blue?
Gram staining highlights different bacteria types through the use of special dyes. It aids in the diagnosis of a specific organism and tells the difference between gram negative and gram positive bacteria. Simple staining is unable to highlight the exact organism.
Which is an example of a dye used in a simple stain?
Methylene blue
What is te value of simple staining?
$3.50.
What information can be obtained from simple staining?
alek mading
Why is simple staining important?
Simple stains are used to highlight microorganisms for the purpose of being able to see their structures and shapes. Simple stains colors commonly used are methylene blue, cargolfuchsin, crystal violet, and safranin.
What is the preparation of smears and simple staining?
smear is the putting and fixing of staining sample on glass slide which is done by first putting the a drop of water on slide and then inoculation is put over it which is then spread slowly in round form by inoculating loop and dry it by very light heat to fix it.Simple staining is the process in which a dye knwon as methylene blue is spread over smear to colour the microbe whcih can be then washed by 70% alcohol so that extra dye can be removed and then the sample is ready to observe under microscope
What does the simple stain tell us about the bacteria?
The result of simple staining of microorganisms is that they can be identified and studied under a microscope.
Simple staining is often nessesary to improve contrast in which microscope?
electron microscope.
Can simple staining be used to identify more than the morphological characteristics of microorganismo?
Yes, it can
What is the purpose of simple staining procedure?
In simple staining the bacterial smear stained with single dye or reagent which bring the distinctive contrast between organism and its background. PROCEDURE: 1 Prepare bacterial smear by placing loopfull culture of bacteria on slide. 2 Allow the slide to dry and heat fix it over flame. Do not heat extreamly, it can demage the shape or structure of bacteria. 3 Now flood the smear with methylene blue for 1-2 minutes. 4 Gently wash the smear with distill water to remove excess stain and dry it slowly with tissue paper. 5 Examine the slide in 100x or oil-immersion for the morphology of bacteria.
What is the principle of capsule staining?
Bacterial capsules are composed of high-molecular-weight polysaccharides and/or polypeptides, and are associated with virulence and biofilm formation. Unfortunately, capsules do not stain well with crystal violet, methylene blue, or other simple stains. This unit describes two methods of capsule staining. The first is a wet-mount method using India ink; the capsule is visualized as a refractile zone surrounding a cell. The second is a direct-staining dry-mount method that precipitates copper sulfate and leaves the capsule as a pale blue zone. Both methods are easily performed within approximately 5 min.
