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In simple staining the bacterial smear stained with single dye or reagent which bring the distinctive contrast between organism and its background. PROCEDURE: 1 Prepare bacterial smear by placing loopfull culture of bacteria on slide. 2 Allow the slide to dry and heat fix it over flame. Do not heat extreamly, it can demage the shape or structure of bacteria. 3 Now flood the smear with methylene blue for 1-2 minutes. 4 Gently wash the smear with distill water to remove excess stain and dry it slowly with tissue paper. 5 Examine the slide in 100x or oil-immersion for the morphology of bacteria.
What else can I help you with?
Which staining technique is used to identify the simple stain(s)?
The staining technique used to identify simple stains is called the simple staining technique.
What can be used for a simple stain procedure?
Perhaps Gram Staining? Steps are as follows: 1. Crystal Violet, 2. Iodine, 3. Decolorizer, 4. Safrinin
What diagnostic procedure used to detect corneal abrasions is known?
Fluorescein staining is the diagnostic procedure used to detect corneal abrasions. In this procedure, a special dye is applied to the eye, which highlights any damaged areas on the surface of the cornea under a cobalt blue light.
What staining procedure will be used to observe bacteria in the sputum sample?
A Gram stain is commonly used to observe bacteria in a sputum sample. This staining procedure helps visualize the bacterial cell wall structure and arrangement, aiding in the identification of different bacterial species.
What is the purpose of staining the smear in malachite green during spore staining?
the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.
What is the best staining procedure to view spirochaetes?
Fontana silver staining.
Which staining technique is used to identify the simple stain(s)?
The staining technique used to identify simple stains is called the simple staining technique.
What staining procedure makes use of pararosanaline?
The Schmorl's stain procedure makes use of pararosaniline as one of its staining components. It is a histological staining method used to highlight cellular structures in tissues.
What is the purpose of spore staining?
not sure
Principle and procedure of histological staining of carbohydrates?
Carbohydrate demonstration methods. Demonstrates Staining mechanisms and technical comments.
What is the purpose of differential staining?
differential staining is a staining technique used to stain colorless bacteria against a dark background.
Which staining procedure uses heat to drive the stain in?
The heat-based staining procedure is called heat fixation. In this process, heat is used to adhere the specimen to the slide, allowing it to withstand the subsequent staining and washing steps without washing away.
What is the four staining procedure in bacteriology?
Gram, Ziel-Nielsen
Why is it important to follow the gram staining procedure?
This is simply important in order to have accurate staining results. If this is not followed, the process of the staining will result to false positives or false negatives.
What can be used for a simple stain procedure?
Perhaps Gram Staining? Steps are as follows: 1. Crystal Violet, 2. Iodine, 3. Decolorizer, 4. Safrinin
What is the purpose of staining?
to detect bacterial stracture
What is te value of simple staining?
$3.50.
