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In simple staining the bacterial smear stained with single dye or reagent which bring the distinctive contrast between organism and its background. PROCEDURE: 1 Prepare bacterial smear by placing loopfull culture of bacteria on slide. 2 Allow the slide to dry and heat fix it over flame. Do not heat extreamly, it can demage the shape or structure of bacteria. 3 Now flood the smear with methylene blue for 1-2 minutes. 4 Gently wash the smear with distill water to remove excess stain and dry it slowly with tissue paper. 5 Examine the slide in 100x or oil-immersion for the morphology of bacteria.
Wiki User
∙ 15y ago
Wiki User
∙ 14y agoIt is the use of single stain or dye to create contrast between bacteria and the background.
Wiki User
∙ 12y agoThe purpose of simple staining is to elucidate the morphology and arrangement of bacterial cells. (Cappuccino, Sherman: Microbiology, a Laboratory Manual).
Wiki User
∙ 9y agoThe function of simple staining is to enhance visual contrast to specimens. This is done to transparent specimens when using a microscope.
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What is the purpose of the heat during the acid fast staining procedure?
It acts as the mordant to soften the mycolic acid so that the stain can penetrate the cell.
What can be used for a simple stain procedure?
Perhaps Gram Staining? Steps are as follows: 1. Crystal Violet, 2. Iodine, 3. Decolorizer, 4. Safrinin
What staining procedure will be used to observe bacteria in the sputum sample?
acid fast staining or Ziel Neelson staining for observing Mycobacteria tuberculosis or Koch bacilli from sputum sample.
What diagnostic procedure used to detect corneal abrasions is known?
fluorescein staining
What is the appearance of capsule after being stain with capsule stain procedure?
capsule does not gets stained it appears to be colourless when stained using manewals staining procedure
What is the best staining procedure to view spirochaetes?
Fontana silver staining.
What is the purpose of the heat during the acid fast staining procedure?
It acts as the mordant to soften the mycolic acid so that the stain can penetrate the cell.
What can be used for a simple stain procedure?
Perhaps Gram Staining? Steps are as follows: 1. Crystal Violet, 2. Iodine, 3. Decolorizer, 4. Safrinin
What is the purpose of spore staining?
not sure
Principle and procedure of histological staining of carbohydrates?
Carbohydrate demonstration methods. Demonstrates Staining mechanisms and technical comments.
What is the purpose of differential staining?
differential staining is a staining technique used to stain colorless bacteria against a dark background.
What is the four staining procedure in bacteriology?
Gram, Ziel-Nielsen
What staining procedure makes use of pararosanaline?
flagellar stain
During the performance of the simple staining procedure you failed to heat fix your EColi smear preparation Upon microscopic examination how would you expect this slide to differ from correct slide?
The bacterial smear will wash away during the staining procedure. This is avoided by heat fixation, during which the bacterial proteins are coagulated and fixed to the glass surface.
Why is it important to follow the gram staining procedure?
This is simply important in order to have accurate staining results. If this is not followed, the process of the staining will result to false positives or false negatives.
What is the purpose of staining?
to detect bacterial stracture
What staining procedure will be used to observe bacteria in the sputum sample?
acid fast staining or Ziel Neelson staining for observing Mycobacteria tuberculosis or Koch bacilli from sputum sample.
