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What staining procedure will be used to observe bacteria in the sputum sample?
Wiki User
∙ 12y ago
acid fast staining or Ziel Neelson staining for observing Mycobacteria tuberculosis or Koch bacilli from sputum sample.
Wiki User
∙ 12y ago
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What is the purpose of bacterial staining?
1. It is rapid method of staining 2. useful in case of sterile samples like CSF pleural fluid etc where no. of pus cells and bacteria will be less
What is the importance of heat fixing the slide before staining?
You heat fix a slide by passing it through a blue flame a couple of times (with th cells facing up). you do this to denature any enzymes that might lyse the cells or interfere with the staining procedure. you also use it kill the organism and to adhere the organism to the slide for staining
What is the preparation of smears and simple staining?
smear is the putting and fixing of staining sample on glass slide which is done by first putting the a drop of water on slide and then inoculation is put over it which is then spread slowly in round form by inoculating loop and dry it by very light heat to fix it.Simple staining is the process in which a dye knwon as methylene blue is spread over smear to colour the microbe whcih can be then washed by 70% alcohol so that extra dye can be removed and then the sample is ready to observe under microscope
Why are contrasting colors important in gram staining?
# Suppose you performed a gram stain on a sample from a pure culture of bacteria and observed a friend of red and purple cocci. Adjacent were not always the same color. What do you conclude? # ## The bacteria was not a pure culture.
What are advantages and disadvantages of staining a sample?
Some cells produce gram-variable results. Some cells produce no results. The age of the culture can influence the results. It has limited usage in environmental biology and is not as good as molecular techniques.
What is the Correct procedure for handling sample of pond water?
place the sample on the microscope slide and observe it.
What is the purpose of bacterial staining?
1. It is rapid method of staining 2. useful in case of sterile samples like CSF pleural fluid etc where no. of pus cells and bacteria will be less
What is the discussion of bacteria smear?
A bacteria smear refers to a technique wherein a small sample of bacteria is spread on a microscope slide for observation. The discussion surrounding bacteria smears typically revolves around examining the morphology, arrangement, and staining characteristics of the bacteria. It is an essential tool for identifying and studying bacterial species.
What is the importance of heat fixing the slide before staining?
You heat fix a slide by passing it through a blue flame a couple of times (with th cells facing up). you do this to denature any enzymes that might lyse the cells or interfere with the staining procedure. you also use it kill the organism and to adhere the organism to the slide for staining
How is trachoma diagnosed?
The doctor will look for the presence of follicles or scarring. He or she will take a small sample of cells from the patient's conjunctivae and examine them, following a procedure called Giemsa staining, to confirm the diagnosis.
What is the preparation of smears and simple staining?
smear is the putting and fixing of staining sample on glass slide which is done by first putting the a drop of water on slide and then inoculation is put over it which is then spread slowly in round form by inoculating loop and dry it by very light heat to fix it.Simple staining is the process in which a dye knwon as methylene blue is spread over smear to colour the microbe whcih can be then washed by 70% alcohol so that extra dye can be removed and then the sample is ready to observe under microscope
What is the importance of gram staining?
Gram staining (or Gram's method) is an empirical method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative) based on the chemical and physical properties of their cell walls. It is a first step to determine the identity of a particular bacterial sample. Gram stains are performed on body fluid or biopsy when infection is suspected. It yields results much more quickly than culture, and is especially important when infection would make an important difference in the patient's treatment and prognosis.
What is the purpose of emulsifying the sample serum in staining procedure?
To promote the cells adhering to the glass slide since they can't be heat fixed. When preparing a capsule stain you have to aseptically add organisms and emulsify with a loop.
What are the most common staining methods in Tissue Engineering. I know HE staining but what else?
H & E staining is good as a primary staining method alone. The selection of a relevant staining method depends on the type of sample you are planning to visualize. Re post with said detail to help you pick the right stain.
Who might use aseptic technique?
microbiologist,biologist and everyone that need to get a clean sample(prevent adding bacteria to the sample).
Suppose you are viewing a smear of a pure sample and you see both pink-red and purple cocci what is going on here?
The sample result is unclear. A number of variables could contribute to mixed results including contaminated equipment, not enough iodine when staining the sample, too much ethanol when rinsing, or not the sample did not heat long enough.
Why are contrasting colors important in gram staining?
# Suppose you performed a gram stain on a sample from a pure culture of bacteria and observed a friend of red and purple cocci. Adjacent were not always the same color. What do you conclude? # ## The bacteria was not a pure culture.
