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1. It is rapid method of staining

2. useful in case of sterile samples like CSF pleural fluid etc where no. of pus cells and bacteria will be less

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What is the purpose of using the primary stain in the process of gram staining?

The purpose of using the primary stain in the process of gram staining is to colorize the bacterial cells, allowing them to be differentiated based on their cell wall composition. This helps to classify the bacteria as either gram-positive or gram-negative.


What bacterial staining technique is it when a basic dye is utilized to stain bacterial cells?

The bacterial staining technique where a basic dye is used to stain bacterial cells is called simple staining. In this technique, the positively charged dye binds to the negatively charged bacterial cell structures, making them more visible under a microscope.


Is endospore staining differencial staining?

Yes, endospore staining is a type of differential staining. It is used to distinguish between bacterial endospores and the vegetative cells of the organism. The endospores appear as green structures against a pink or red background when using the Schaeffer-Fulton staining technique.


What is the purpose of staining the smear in malachite green during spore staining?

the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.


What staining procedure will be used to observe bacteria in the sputum sample?

A Gram stain is commonly used to observe bacteria in a sputum sample. This staining procedure helps visualize the bacterial cell wall structure and arrangement, aiding in the identification of different bacterial species.

Related Questions

What is the purpose of staining?

to detect bacterial stracture


What is the purpose of albert staining?

to detect bacterial stracture


What is thee purpose of adding methylene blue to the specimen?

Adding methylene blue to a slide will stain animal cells and make the nuclei more visible.


What is the purpose of using the primary stain in the process of gram staining?

The purpose of using the primary stain in the process of gram staining is to colorize the bacterial cells, allowing them to be differentiated based on their cell wall composition. This helps to classify the bacteria as either gram-positive or gram-negative.


What bacterial staining technique is it when a basic dye is utilized to stain bacterial cells?

The bacterial staining technique where a basic dye is used to stain bacterial cells is called simple staining. In this technique, the positively charged dye binds to the negatively charged bacterial cell structures, making them more visible under a microscope.


Is endospore staining differencial staining?

Yes, endospore staining is a type of differential staining. It is used to distinguish between bacterial endospores and the vegetative cells of the organism. The endospores appear as green structures against a pink or red background when using the Schaeffer-Fulton staining technique.


What is the purpose of staining the smear in malachite green during spore staining?

the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.


Bacterial smears are fixed before staining to?

Bacterial smears are fixed before staining to adhere the bacteria to the slide and kill the bacteria, which helps to prevent them from washing away during the staining process. Additionally, fixing the bacteria helps to preserve their cellular structures and prevent distortion during staining.


What is the purpose of spore staining?

not sure


What is the purpose of differential staining?

differential staining is a staining technique used to stain colorless bacteria against a dark background.


What staining procedure will be used to observe bacteria in the sputum sample?

A Gram stain is commonly used to observe bacteria in a sputum sample. This staining procedure helps visualize the bacterial cell wall structure and arrangement, aiding in the identification of different bacterial species.


What is the purpose of heating slide with carbol fuchsin?

Heating the slide with carbol fuchsin helps to penetrate the bacterial cell wall and enhance the staining process. This allows the dye to better adhere to the bacterial cells, making them easier to visualize under the microscope.