1. It is rapid method of staining
2. useful in case of sterile samples like CSF pleural fluid etc where no. of pus cells and bacteria will be less
to identification which bacteria present/found in given sample.
Endospore staining is a differential stain used to detect the presence and location of spores in bacterial cells.
The purpose of heat fixing the bacteria to the slide is so during the gram staining procedure the bacteria doesn't wash off. If you didn't heat fix the bacteria to the slide, it would not stay on the slide.
A basic dye used in gram staining is crystal violet.
Leishman staining is used for staining blood in microscopy and its purpose is to both identify and differentiate trypanosomas, leucocytes and malaria parasites. Giesma staining is used to stain DNA region, specifically chromosomes in order to locate aberrations like rearrangement and translocations.
In simple staining the bacterial smear stained with single dye or reagent which bring the distinctive contrast between organism and its background. PROCEDURE: 1 Prepare bacterial smear by placing loopfull culture of bacteria on slide. 2 Allow the slide to dry and heat fix it over flame. Do not heat extreamly, it can demage the shape or structure of bacteria. 3 Now flood the smear with methylene blue for 1-2 minutes. 4 Gently wash the smear with distill water to remove excess stain and dry it slowly with tissue paper. 5 Examine the slide in 100x or oil-immersion for the morphology of bacteria.
to detect bacterial stracture
to detect bacterial stracture
Adding methylene blue to a slide will stain animal cells and make the nuclei more visible.
Endospore staining is a differential stain used to detect the presence and location of spores in bacterial cells.
The purpose of heat fixing the bacteria to the slide is so during the gram staining procedure the bacteria doesn't wash off. If you didn't heat fix the bacteria to the slide, it would not stay on the slide.
not sure
differential staining is a staining technique used to stain colorless bacteria against a dark background.
A basic dye used in gram staining is crystal violet.
affix the cells to the slide and to kill bacteria
simple
In Gram staining procedure during bacterial staining , iodine forms a complex with crystal violet stain which stains Gram positive bacteria blue to violet .
Capsules may be revealed by methods such as Maneval's method. This method utilizes negative staining, where the background is stained revealing an unstained structure: the bacterial capsule.