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What else can I help you with?
Why should the the slide be flooded with a strong carbol fuschin?
Flooding the slide with strong carbol fuchsin helps in staining the mycobacteria in acid-fast staining techniques by allowing the stain to penetrate the mycolic acid layer in the cell wall. This improves the sensitivity of detecting acid-fast bacilli in the sample, making them more visible under the microscope. Additionally, the carbol fuchsin helps in differentiating acid-fast bacteria from other bacteria that may be present in the sample.
Difference between hot method and cold method of acid fast staining?
The hot method involves heating the stained slide with acid-alcohol to help penetrate the waxy cell wall of acid-fast bacteria, while the cold method does not require this heating step. The hot method typically yields faster results but may also lead to more fading of stain colors compared to the cold method. Both methods rely on the differential staining properties of acid-fast bacteria, such as Mycobacterium species, and non-acid-fast bacteria.
Describe the mechanism of action for acid-fast stain?
ACID FAST Os Contains waxlike lipoidal material affecting staining quality. Carbolfuchsin is primary stain. Acid fast organisms resist decolorization with acid alcohol. After decolorization, methyelene blue is added to organisms to counterstain any material that is not acid fast. It is used for examination of Mycobacterium. The principle of staining is depend on the resistance of this type of bacteria to decolarization by acid alcohol, because the cell wall contain waxy material (mycolic acid) which prevent the removal of carbol fuchsin from the cell. Staining Procedure 1. Flood the slide with strong carbol fuchsin and heat until steam rises (but do not boil). 2. After 3-4 min apply more heat until rises again; do not let the stain dry on the slide. 3. About 5-7 min after the first application of heat washes the slide thoroughly under running water. 4. Decolourize in acid-alcohol until all traces of red have disappeared from the film. Decolourization should not be attempted in one stage; there should be intermittent washings in water and re-application of acid-alcohol. 5. Wash well with water and counter stain with Methylene blue for 1 min. 6. Wash and stand end to drain. 7. Acid-fast organisms are red, other organisms are blue.
Why must smear on the slide be completely dry before heat fixation?
Slight heating helps in fixing the cells on to the surface of the glass slide
What problems arise what the slide is heated in a flame?
Prolonged heating of the slide can cause the slide to shatter.
Why should the the slide be flooded with a strong carbol fuschin?
Flooding the slide with strong carbol fuchsin helps in staining the mycobacteria in acid-fast staining techniques by allowing the stain to penetrate the mycolic acid layer in the cell wall. This improves the sensitivity of detecting acid-fast bacilli in the sample, making them more visible under the microscope. Additionally, the carbol fuchsin helps in differentiating acid-fast bacteria from other bacteria that may be present in the sample.
Why steam heating is done in acid fast staining and not heat fixing?
Actually, both methods are used during the staining procedure (steam & heat fix). Initially, the organism is heat fixed to the slide to prevent the organism from being washed off during subsequent steps. Later in the procedure, the slide with the heat fixed organism is steamed to make the cell wall a little more penetrable - allowing the stain to enter the cell wall.
Difference between hot method and cold method of acid fast staining?
The hot method involves heating the stained slide with acid-alcohol to help penetrate the waxy cell wall of acid-fast bacteria, while the cold method does not require this heating step. The hot method typically yields faster results but may also lead to more fading of stain colors compared to the cold method. Both methods rely on the differential staining properties of acid-fast bacteria, such as Mycobacterium species, and non-acid-fast bacteria.
What problems can arise when the slide is heated in a flame?
Heating the slide in a flame can cause it to crack or break due to uneven expansion or thermal shock. This can lead to sample loss and potential injury from hot glass shards. Additionally, heating the slide too quickly or with excessive heat can degrade the sample being analyzed.
What is the purpose of a point?
Purpose of power point is to create a slide. A slide is a presentation for a business meeting or group.
Describe the mechanism of action for acid-fast stain?
ACID FAST Os Contains waxlike lipoidal material affecting staining quality. Carbolfuchsin is primary stain. Acid fast organisms resist decolorization with acid alcohol. After decolorization, methyelene blue is added to organisms to counterstain any material that is not acid fast. It is used for examination of Mycobacterium. The principle of staining is depend on the resistance of this type of bacteria to decolarization by acid alcohol, because the cell wall contain waxy material (mycolic acid) which prevent the removal of carbol fuchsin from the cell. Staining Procedure 1. Flood the slide with strong carbol fuchsin and heat until steam rises (but do not boil). 2. After 3-4 min apply more heat until rises again; do not let the stain dry on the slide. 3. About 5-7 min after the first application of heat washes the slide thoroughly under running water. 4. Decolourize in acid-alcohol until all traces of red have disappeared from the film. Decolourization should not be attempted in one stage; there should be intermittent washings in water and re-application of acid-alcohol. 5. Wash well with water and counter stain with Methylene blue for 1 min. 6. Wash and stand end to drain. 7. Acid-fast organisms are red, other organisms are blue.
What is the purpose of the title slide?
To introduce what it is your about to talk about(:
What is the purpose of the drawer slide rear mounting bracket?
The purpose of the drawer slide rear mounting bracket is to provide support and stability to the drawer slide, allowing it to smoothly glide in and out of the cabinet or furniture piece.
Why must smear on the slide be completely dry before heat fixation?
Slight heating helps in fixing the cells on to the surface of the glass slide
What is the purpose of slide master?
See you next tuesday
Function of the counterstain in acid-fast?
The function of a counterstain in acid-fast stains is to dye the non acid-fast bacteria cells a different color than the acid-fast cells. With two different colors present on a slide, the contrast between the two types (acid-fast and non acid-fast) is more distinct. The more distinct view of cells will assist in observations of a slide.
What is gram stain reaction for Borrelia Burgdoferi?
when stained with Gram stain Borrelia take up the counter stain which is carbol fuchsin or safranin and they appear as Gram negative spiral rods in gram film. In order to stain them the time required for staining them is little bit more as compared to normal gram staining. The initial steps are the same but once you apply the counter stain leave it for a while may be 5-10 mins depending upon the strength of counter stain. After washing the slide and drying once can see them on oil immersion lense.
