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when stained with Gram stain Borrelia take up the counter stain which is carbol fuchsin or safranin and they appear as Gram negative spiral rods in gram film. In order to stain them the time required for staining them is little bit more as compared to normal gram staining. The initial steps are the same but once you apply the counter stain leave it for a while may be 5-10 mins depending upon the strength of counter stain. After washing the slide and drying once can see them on oil immersion lense.
What else can I help you with?
Do acid-fast bacteria stain gram-negative when subjected to the gram stain?
No, acid-fast bacteria do not stain gram-negative when subjected to the gram stain.
Theories of principle of gram stain reactions?
Gram stain
What does a gram reaction of a cell refer to?
the Gram reaction is based on the structure of the bacterial cell wall. In Gram-positive bacteria, the dark purple crystal violet stain is retained by the thick layer of peptidoglycan which forms the outer layer of the cell. In Gram-negative bacteria, the thin peptidoglycan layer in the periplasm does not retain the dark stain, and the pink safranin counter stain stains the peptidoglycan layer. In other word,the gram reaction refers to how the cells reacts to the gram-staining process.
What is the counter or secondary stain used in the Gram stain procedure?
The counter or secondary stain used in the Gram stain procedure is safranin.
What is the difference between Jensen's version ang hucker's modification in gram staining based on reagents and gram reaction to fungi?
What is modified in Jensen's modification of Gram stain? Jensen's modification: This method involves use to methyl violet as primary stain, iodine and potassium iodide in water as mordant, absolute alcohol as decolorizer and neutral red as counterstain
Is the disease Lyme disease gram stain positive?
Spirochetes are not considered Gram positive or Gram negative. They tend to stain pink due to the last safrinin dye in the staining process, but its cell wall structure should make it Gram negative.
What is the gram stain reaction of strep throat?
Streptococcus pyogenes is gram positive bacteria.
Gram stain of lymes disease?
Lyme Disease is caused by Borrelia burgdorferi. This is a spirochete bacterium with a gram negative cell wall structure. These organisms are best viewed by dark-field microscopy.
What is the shape of borrelia recurrentis?
borrelia recurrentis is a spiral shape gram -ve bacteria
Does pH influence the gram stain reaction?
pH does not directly influence the Gram stain reaction. The Gram stain differentiates between bacterial cell wall structures based on their ability to retain or release crystal violet dye during the staining process. The process is not significantly affected by small changes in pH.
Do acid-fast bacteria stain gram-negative when subjected to the gram stain?
No, acid-fast bacteria do not stain gram-negative when subjected to the gram stain.
Theories of principle of gram stain reactions?
Gram stain
What is the gram stain for cholera?
Gram Negative
What three types of data are obtained from the gram stain?
The three types of data obtained from a Gram stain are cell morphology (shape and size of the cells), cell arrangement (how cells are grouped together), and Gram reaction (whether cells are Gram-positive or Gram-negative based on their cell wall composition).
What is the different reaction of Gram-negative and Gram-positive bacteria to Gram's stain testing?
Gram-positive bacteria retain the crystal violet stain and appear purple when stained with Gram's stain, because the thick peptidoglycan layer in their cell wall traps the dye. On the other hand, Gram-negative bacteria do not retain the crystal violet stain and appear red or pink after the addition of a counterstain like safranin, due to their thin peptidoglycan layer which is unable to retain the dye.
What does a gram reaction of a cell refer to?
the Gram reaction is based on the structure of the bacterial cell wall. In Gram-positive bacteria, the dark purple crystal violet stain is retained by the thick layer of peptidoglycan which forms the outer layer of the cell. In Gram-negative bacteria, the thin peptidoglycan layer in the periplasm does not retain the dark stain, and the pink safranin counter stain stains the peptidoglycan layer. In other word,the gram reaction refers to how the cells reacts to the gram-staining process.
Can a Gram stain a prion?
Protists are often stained using a silver stain, not a Gram stain.
