What is modified in Jensen's modification of Gram stain?
Jensen's modification: This method involves use to methyl violet as primary stain, iodine and potassium iodide in water as mordant, absolute alcohol as decolorizer and neutral red as counterstain
Common sources of gram staining errors include improper decolorization, incorrect timing during the staining process, over- or under-fixation of the bacterial cells, poor quality of reagents, and using old or degraded bacterial cultures. These factors can lead to inaccurate results where Gram-positive bacteria appear as Gram-negative or vice versa.
A Gram-variable reaction may occur when the bacteria have an atypical cell wall structure, making it difficult to predict whether they will appear as Gram-positive or Gram-negative under a microscope. This variability in staining can be caused by factors such as age of the culture, cell wall composition, or bacterial species.
Mycobacteria are typically Gram-positive, but their cell wall structure is unique and contains high lipid content, making them resistant to Gram staining. They are best visualized using acid-fast staining methods, such as the Ziehl-Neelsen or Kinyoun stains.
Leishman staining is used for staining blood in microscopy and its purpose is to both identify and differentiate trypanosomas, leucocytes and malaria parasites. Giesma staining is used to stain DNA region, specifically chromosomes in order to locate aberrations like rearrangement and translocations.
1- What_is_the_different_staining_technique_in_virology2- What are the diffrent stain in micro for virus ?
It sometimes require additional chemical reagents to produce the desired action.
A Coplin jar is used in laboratory settings to hold and process multiple microscope slides at the same time. It is commonly used for staining procedures, such as the Gram stain, where multiple slides need to be immersed in various staining reagents simultaneously.
Common sources of gram staining errors include improper decolorization, incorrect timing during the staining process, over- or under-fixation of the bacterial cells, poor quality of reagents, and using old or degraded bacterial cultures. These factors can lead to inaccurate results where Gram-positive bacteria appear as Gram-negative or vice versa.
Mordant reagents are used in staining techniques to help bind dyes to specific structures. Different mordants are needed for different types of dyes and tissue components. For example, in Gram staining, the mordant reagent is iodine, which helps bind the crystal violet dye to the bacterial cell wall.
Ethanol hydration in immunohistochemistry is used to rehydrate tissue sections that have been dehydrated during the staining process. This step allows for better penetration of antibodies and reagents into the tissue, improving the overall staining quality and specificity.
A Gram-variable reaction may occur when the bacteria have an atypical cell wall structure, making it difficult to predict whether they will appear as Gram-positive or Gram-negative under a microscope. This variability in staining can be caused by factors such as age of the culture, cell wall composition, or bacterial species.
Mycobacteria are typically Gram-positive, but their cell wall structure is unique and contains high lipid content, making them resistant to Gram staining. They are best visualized using acid-fast staining methods, such as the Ziehl-Neelsen or Kinyoun stains.
Leishman staining is used for staining blood in microscopy and its purpose is to both identify and differentiate trypanosomas, leucocytes and malaria parasites. Giesma staining is used to stain DNA region, specifically chromosomes in order to locate aberrations like rearrangement and translocations.
Gram staining is used to identify whether a bacterium is gram positive or gram negative. Slides can be dried using filter paper or tissues. The technique is based on the reaction of stain that happens with the membrane of bacteria.
In Gram staining, some bacterial species will become Gram variable meaning they produce a false reaction for the following reasons: decolrized too long, too young/old of culture, stained too long. Also, time is important simply because you don't want to over or under stain. You won't be able to see your specimen as well.
REGRESSIVE STAINING. In a regressive stain, the tissue is first over stained and then partially decolorized. Differentiation is usually controlled visually by examination with a microscope. When regressive staining is employed, a sharper degree of differentiation is obtained than with progressive staining .PROGRESSIVE STAINING. In progressive staining, once the dye is taken up by the tissue it is not removed. Differentiation in progressive staining relies solely on the selective affinity of dyes for different tissue elements. The tissue is left in the dye solution only until it retains the desired amount of coloration.
Thomas Francis McNamara has written: 'Iodine and the quantitative gram reaction' -- subject(s): Iodine, Stains and staining (Microscopy)