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What is the protocol of leifson method in flagella staining?
The Leifson staining method is a technique used to stain flagella. The protocol involves fixing the bacteria onto a slide, flooding with tannic acid, applying basic fuchsin, rinsing with distilled water, and then rinsing with copper sulfate. This staining technique highlights the flagella, making them visible under the microscope.
Can use carbolfuchsin as a counterstain?
Carbolfuchsin can be used as a counterstain in certain staining techniques, particularly in the acid-fast staining method used to detect acid-fast bacteria like Mycobacterium tuberculosis. It helps to differentiate acid-fast bacteria, which retain the primary stain (carbolfuchsin), from non-acid-fast bacteria which are counterstained with a contrasting color.
What is the difference between amino acid and nucleic acids?
An amino acid is the monomer of proteins, and a nucleic acid is genetic material.
What ar the component of acid fast staining?
Periodic acid-Schiff (PAS) is a staining method used in histology and pathology. This method is primarily used to identify glycogen in tissues. The reaction of periodic acid selectively oxidizes the glucose residues, creates aldehydes that react with the Schiff reagent and creates a purple-magenta color. A suitable basic stain is often used as a counterstain.Source: http://en.wikipedia.org/wiki/Periodic_acid-Schiff_stain
What is the difference between one amino acid and another is the?
Assuming that you are talking about the amino acids that are synthesized from tRNA, the difference between one amino acid and another is the 3 letter RNA sequence that calls for the acid: UUG would call for one acid, while AAC will most likely call for another.
What is the difference between a lead-acid accumulator and a lead-acid battery?
There is no difference between lead acid accumulator and lead acid battery.
What is the difference between a hydrocarbon and a fatty acid?
The difference between a hydrocarbon and a fatty acid is that a fatty acid is a hydrocarbon chain with a carboxylic acid head.
What is staining in a pool?
Acid
What stain hemoglobin casts dark-blue to blue-black in puchtler's method for hemoglobin?
Hematoxylin stain can cast hemoglobin dark-blue to blue-black in Puchtler's method for hemoglobin staining. This method involves staining with hematoxylin followed by differentiation in acid alcohol and a counterstain in eosin.
Is the acid fast staining result positive or negative for the sample?
The acid-fast staining result for the sample is positive.
What is the protocol of leifson method in flagella staining?
The Leifson staining method is a technique used to stain flagella. The protocol involves fixing the bacteria onto a slide, flooding with tannic acid, applying basic fuchsin, rinsing with distilled water, and then rinsing with copper sulfate. This staining technique highlights the flagella, making them visible under the microscope.
Can use carbolfuchsin as a counterstain?
Carbolfuchsin can be used as a counterstain in certain staining techniques, particularly in the acid-fast staining method used to detect acid-fast bacteria like Mycobacterium tuberculosis. It helps to differentiate acid-fast bacteria, which retain the primary stain (carbolfuchsin), from non-acid-fast bacteria which are counterstained with a contrasting color.
Discuss the different methods of acid-fast staining?
Ziehl-Neelsen Technique. also know as the Hot stain. application of heat during staining for the greater penetration of the primary stain which is the Carbolfushin. Kinyoun techinique. also know as the Cold stain. Acid fast organisms can also be visualized by Flourescence Microscopy using specific flourescent dyes such as Auramine and Rhodamine. :D -iampol
What is the difference between amino acid and a nucleic acid?
An amino acid is the monomer of proteins, and a nucleic acid is genetic material.
What is difference between an amino acid and a nucleic acid?
An amino acid is the monomer of proteins, and a nucleic acid is genetic material.
Difference between vinegar and acetic acid?
no differnce
What kind of stain do you stain Mycobacterium with?
For Mycobacterium you will use the Acid-fast staining technique. There are two different methods of stainging: 1) Ziehl-Neelsen Method and 2) Kinyoun Method.1) The Ziel-Neelsen method uses a primary stain of Carbol Fuchsin dye that must be steam treated, rinsed with acid alcohol wash, and a secondary stain of Methylene Blue.2) The Kinyoun Method uses a primary stain of Kinyoun Carbol Fuchsin dye that is not steam treated. An acid alcohol wash is applied and a secondary dye of Brilliant Green. This technique is called "cold staining".The mycolic acid within the Mycobacterium cell membrane has a high affinity for the Carbol Fuchsin dyes.
