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Difference between hot method and cold method of acid fast staining?
Wiki User
∙ 15y ago
The hot method uses heat to force the primary stain (carbol fuchsin) into the cell wall, while the cold method uses a detergent to cause the stain to penetrate the wall.
Wiki User
∙ 15y ago
Wiki User
∙ 10y agocret
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Difference between borax boric acid?
No. No difference. Boric Acid is actually in a powder form.
What is the difference between amino acid and nucleic acids?
An amino acid is the monomer of proteins, and a nucleic acid is genetic material.
How do you decolourise schiffs reagent?
With periodic acid during the steps of staining slides.
What ar the component of acid fast staining?
Periodic acid-Schiff (PAS) is a staining method used in histology and pathology. This method is primarily used to identify glycogen in tissues. The reaction of periodic acid selectively oxidizes the glucose residues, creates aldehydes that react with the Schiff reagent and creates a purple-magenta color. A suitable basic stain is often used as a counterstain.Source: http://en.wikipedia.org/wiki/Periodic_acid-Schiff_stain
Explain the difference between the solubility behaviors of glycerol and triglycerides?
Explain the difference between the solubility behaviors of glycerol,fatty acid and triglycerides
What is staining in a pool?
Acid
What is the difference between a lead-acid accumulator and a lead-acid battery?
There is no difference between lead acid accumulator and lead acid battery.
What is the difference between a hydrocarbon and a fatty acid?
The difference between a hydrocarbon and a fatty acid is that a fatty acid is a hydrocarbon chain with a carboxylic acid head.
What is the difference between Oleic acid and Erucic acid?
Banana's
What is the difference between carbonic acid and acid rain?
the difference between them is that carbonic acid involve carboxyl compound and water while acid rain involves acidic compound and water.
Difference between borax boric acid?
No. No difference. Boric Acid is actually in a powder form.
What is the difference between acetylsalicylic acid and salicylic acid?
has a pH of 2
Whats the difference between acid and LSD?
Acid is slang for Lysergic acid diethylamide (LSD).
What is the difference between nitrous acid and nitric acid?
both have different valency
Difference between a weak and a strong acid involves the acid's ability to?
ionize
What are the consequences of Ziehl-Neelsen staining?
The Ziehl-Neelsen stain is also known as the acid-fast stain. It contains sulfuric acid, and is used to identify acid-fast bacteria, or bacteria resistant to decolorization by acids from staining.
What kind of stain do you stain Mycobacterium with?
For Mycobacterium you will use the Acid-fast staining technique. There are two different methods of stainging: 1) Ziehl-Neelsen Method and 2) Kinyoun Method.1) The Ziel-Neelsen method uses a primary stain of Carbol Fuchsin dye that must be steam treated, rinsed with acid alcohol wash, and a secondary stain of Methylene Blue.2) The Kinyoun Method uses a primary stain of Kinyoun Carbol Fuchsin dye that is not steam treated. An acid alcohol wash is applied and a secondary dye of Brilliant Green. This technique is called "cold staining".The mycolic acid within the Mycobacterium cell membrane has a high affinity for the Carbol Fuchsin dyes.
