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Ziehl-Neelsen Technique.
also know as the Hot stain.
application of heat during staining for the greater penetration of the primary stain which is the Carbolfushin.
Kinyoun techinique.
also know as the Cold stain.
Acid fast organisms can also be visualized by Flourescence Microscopy using specific flourescent dyes such as Auramine and Rhodamine.
:D
-iampol
What else can I help you with?
Can simple staining techniques be used to identify more than the morphological characteristics of microorganisms?
Yes, you can determine the arrangement of the micoorganism such as chain, random, or spiral as the simple staining allows you to obtained a distinctive contrast between background and its organisms being observed.
Can you see organelle in microscope?
Yes, organelles such as nucleus, mitochondria, and chloroplasts can be seen through a microscope using various staining techniques to enhance their visibility. The use of different microscopy methods, such as electron microscopy, can provide higher resolution images of organelles within a cell.
What is a primary stain?
If your talking about Acid Fast staining (aka Ziehl-Neelsen staining), after the addition of the primary stain (carbol fuchsin) heat is applied in order to facilitate proper staining. Bacteria such as Mycobacterium contain large amounts of lipid substances within their cell wall called mycolic acids. These acids resist staining by ordinary methods such as gram staining (where heat is not applied after primary staining). On application of heat, the stain carbol fuschin penetrates the cell wall and stains the cells pink. Following the secondary staining (methylene blue) the acid fast positive cells appear pink while others are stained blue. Endospore staining is yet another staining technique where heat is applied after primary staining (malachite green). In this case the spores are impermeable to stains, hence heating favours proper permeation of stain. Endospores appear green while vegetative cells appear red (secondary stain saffranine). Not all staining procedures involve applying heat after primary staining. However, heat is applied before even beginning the staining procedure. This is called heat fixing, where the cells are fixed so that they are not washed away during the subsequent washing steps.
What organelles can be seen under microscope?
Organelles that can be seen under a microscope include the nucleus, mitochondria, endoplasmic reticulum, golgi apparatus, lysosomes, and chloroplasts (in plant cells). These organelles can be visualized using different staining techniques and microscopy methods, such as light microscopy or electron microscopy.
What are the gram reaction of mycobacterium?
Mycobacteria are typically Gram-positive, but their cell wall structure is unique and contains high lipid content, making them resistant to Gram staining. They are best visualized using acid-fast staining methods, such as the Ziehl-Neelsen or Kinyoun stains.
Is serratia marcescens acid-fast?
No, Serratia marcescens is a Gram-negative bacterium and is not acid-fast. Acid-fast staining technique is used to detect organisms like Mycobacterium tuberculosis, which have a waxy lipid layer in their cell wall that resists staining by conventional methods.
Principle and procedure of histological staining of carbohydrates?
Carbohydrate demonstration methods. Demonstrates Staining mechanisms and technical comments.
Discuss methods of communication in management?
how to discuss different communication methods well, I'm looking for the same answer and cant find it anywhere, any help would be great thanks, this is the question worth 8 marks; Karen has been asked to manage an international project for her company based in London. They want her to create a presentation based on information from other offices in Paris, Madrid and Tokyo. Discuss the different methods that she could use to coordinate with the other offices.
What are the most common staining methods in Tissue Engineering. I know HE staining but what else?
H & E staining is good as a primary staining method alone. The selection of a relevant staining method depends on the type of sample you are planning to visualize. Re post with said detail to help you pick the right stain.
Discuss the merits and drawbacks of the point-by-point and block methods?
The point method measure price elasticity of demand at a different point. Its a refund.
What were the purification methods used in the olden days?
Discuss the purification methods used in the olden days
What is a histology special stain?
A histology special stain is a type of staining technique used in histology to selectively highlight specific structures or substances in tissue samples that may not be visible with standard staining methods. These stains help in visualizing different components of cells or tissues, aiding in the identification and diagnosis of various diseases.
How can a positive result occur during capsule staining?
Capsules may be revealed by methods such as Maneval's method. This method utilizes negative staining, where the background is stained revealing an unstained structure: the bacterial capsule.
Why don't capsules pick up the stain?
Capsules are made of polysaccharides and/or polypeptides that have no net charge. Most dyes used do have a net charge. Therefore, capsules cannot bind to charged dyes and do not stain as a result. Capsules may be revealed by methods such as Maneval's method. This method utilizes negative staining, where the background is stained revealing an unstained structure of interest: the bacterial capsule.
Different methods of paraghaph developing?
different methods of paragraph development
Why is immunohistochemistry stain working on the control tissue but not the experimental tissue?
There could be multiple reasons why immunohistochemistry staining may work on control tissue but not on experimental tissue, such as differences in antigen expression, tissue processing methods, or antibody specificity. It's important to carefully review and troubleshoot the staining protocol, as well as consider factors like fixation and processing conditions that may affect the staining outcome. Additionally, confirming the presence of the antigen of interest in the experimental tissue using alternative methods can help identify potential issues with the immunohistochemistry staining.
Explain three different methods in java?
There are three different methods /functions in java are there : 1)computational methods.2)manipulative methods.3)procedural methods.
