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Why is immunohistochemistry stain working on the control tissue but not the experimental tissue?
There could be multiple reasons why immunohistochemistry staining may work on control tissue but not on experimental tissue, such as differences in antigen expression, tissue processing methods, or antibody specificity. It's important to carefully review and troubleshoot the staining protocol, as well as consider factors like fixation and processing conditions that may affect the staining outcome. Additionally, confirming the presence of the antigen of interest in the experimental tissue using alternative methods can help identify potential issues with the immunohistochemistry staining.
What else can I help you with?
What tissue makes up organs that send messages to control the body?
Nervous Tissue(s)
What kind of stain would you use to visualize Sars?
A common stain used to visualize SARS-CoV-2 (the virus responsible for COVID-19) is hematoxylin and eosin (H&E) stain for histopathological examination of tissue samples. Additionally, immunohistochemistry staining using specific antibodies against viral antigens can also be employed to visualize SARS-CoV-2 in tissue samples.
What is serveral tissue working togethor to perform a complex task are called?
Several tissues working together to perform a complex task are called an organ. Organs are structures made up of different types of tissues that collaborate to carry out specific functions in an organism. For example, the heart is an organ composed of muscle tissue, connective tissue, and nervous tissue, all working together to pump blood throughout the body.
What is a structure made of several kinds of tissue working together in one function?
A structure made of several kinds of tissue working together to perform a specific function is called an organ. For example, the heart is an organ composed of muscle tissue, connective tissue, and nervous tissue, all collaborating to pump blood throughout the body. Each type of tissue contributes to the overall function and efficiency of the organ, highlighting the complexity of biological systems.
Group of cells of the same kind working together?
A group of plant cells working together forms plant tissue.
What is the purpose of ethanol hydration in immunohistochemistry?
Ethanol hydration in immunohistochemistry is used to rehydrate tissue sections that have been dehydrated during the staining process. This step allows for better penetration of antibodies and reagents into the tissue, improving the overall staining quality and specificity.
What is the purpose of Xylene in immunohistochemistry?
Xylene is an organic solvent which is miscible with alcohol and wax. In histochemistry alcohol is used to dehydrate tissue sections and then this alcohol is further replaced by xylene (being miscible with alcohol) in a process called as clearing. After this DPX is used to mount cover slips on the sections. One more use of xylene is to dissolve wax which is used to make sections of tissues. Hence, we see that xylene is a clearing agent capable of dissolving wax as well as alcohol.
What is immunohistochemistry?
Immunohistochemistry or IHC refers to the process of localizing proteins in cells of a tissue section exploiting the principle of antibodies binding specifically to antigens in biological tissues. [1] It takes its name from the roots "immuno," in reference to antibodies used in the procedure, and "histo," meaning tissue (compare to immunocytochemistry). Immunohistochemical staining is widely used in the diagnosis of abnormal cells such as those found in cancerous tumors. Specific molecular markers are characteristic of particular cellular events such as proliferation or cell death (apoptosis). IHC is also widely used in basic research to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue.
What is immunohisochemistry?
Immunohistochemistry is a lab technique where a slide is made from a slice of tissue. It is treated with an antibody against the protein of interest that is made to bind to the protien with one end and and have a chemical reaction on the other. The chemical reaction can then be used to make the protein visible under the microscope.
What is tissue organ organ system and organism?
The difference between organ system and tissue is that organs are made of many layers of tissue working together and a organ system is many organs working together as a system.
What is a tissue composed of?
similar cells working together
What are Two or more tissue working together?
The answer is a organ!!!!!!!!!!!!!!!!!!!
What is the control in an experiment to demonstrate osmosis?
If you are doing the experiment with potato or similar (other plant tissue) then you boil the potato you are taking the sample from before you use it, to shoe that the plant tissue needs to be alive for the experiment to work.
How does the organization of an organ differ from that of tissue?
An organ is made of different tissues working together to perform a body function. In contrast, tissue is groups of cells working together.
What tissue makes up organs that send messages to control the body?
Nervous Tissue(s)
What tissue makes up the organs the send messages to control the body?
Nervous Tissue(s)
What tissue makes up organs that send messages to control body?
Nervous Tissue(s)
