0
Immunohistochemistry or IHC refers to the process of localizing proteins in cells of a tissue section exploiting the principle of antibodies binding specifically to antigens in biological tissues. [1] It takes its name from the roots "immuno," in reference to antibodies used in the procedure, and "histo," meaning tissue (compare to immunocytochemistry). Immunohistochemical staining is widely used in the diagnosis of abnormal cells such as those found in cancerous tumors. Specific molecular markers are characteristic of particular cellular events such as proliferation or cell death (apoptosis). IHC is also widely used in basic research to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue.
Wiki User
∙ 15y ago
Add your answer:
Earn +20 pts
Full name of DPX in immunohistochemistry?
Distyrene Plasticizer Xylene
Where can you find information on immunohistochemistry?
You can find information on immunohistochemistry in scientific journals, textbooks on molecular biology or histology, and online resources such as databases or websites of research institutes. Additionally, universities often offer courses or workshops on immunohistochemistry for those looking to learn more about the technique.
What has the author Paul Hirsch written?
Paul Hirsch has written: 'Immunochemische Studien' -- subject(s): Immunohistochemistry
What is the purpose of ethanol hydration in immunohistochemistry?
Its used for dehydration after the xylene process at the beginning and end of the procedure before the coverslip is placed on the slide.
What is BioCare Medical most famous for consumers?
BioCare Medical provides high quality immunohistochemistry and molecular pathology products. They have ancillaries, antibodies, and equipment for detection.
What has the author Mehrdad Nadji written?
Mehrdad Nadji has written: 'Immunoperoxidase techniques' -- subject(s): Diagnosis, Handbooks, manuals, Immunoenzyme technics, Immunoenzyme technique, Immunohistochemistry, Neoplasms, Tumors
What is immunohisochemistry?
Immunohistochemistry is a lab technique where a slide is made from a slice of tissue. It is treated with an antibody against the protein of interest that is made to bind to the protien with one end and and have a chemical reaction on the other. The chemical reaction can then be used to make the protein visible under the microscope.
What has the author Hossein M Yazdi written?
Hossein M. Yazdi has written: 'Diagnostic immunocytochemistry and electron microscopy' -- subject(s): Cancer, Cytodiagnosis, Diagnosis, Electron microscopic, Electron microscopy, Immunocytochemistry, Immunohistochemistry, Laboratory Diagnosis, Methods, Needle biopsy
What has the author Barbara Symonds Beltz written?
Barbara Symonds Beltz is the author of "The Little Dragon Who Couldn't Roar," a children's book about self-acceptance and friendship. She has also written works related to personal development and spirituality.
What is the purpose of Xylene in immunohistochemistry?
Xylene is an organic solvent which is miscible with alcohol and wax. In histochemistry alcohol is used to dehydrate tissue sections and then this alcohol is further replaced by xylene (being miscible with alcohol) in a process called as clearing. After this DPX is used to mount cover slips on the sections. One more use of xylene is to dissolve wax which is used to make sections of tissues. Hence, we see that xylene is a clearing agent capable of dissolving wax as well as alcohol.
The difference between IHC and western blot?
Immunohistochemistry (IHC) is a method used to visualize protein expression in tissue samples using antibodies and staining techniques, whereas western blot is a technique that detects and quantifies specific proteins in a sample using antibodies and gel electrophoresis. IHC is used to analyze protein expression in tissue samples, while western blot is used to analyze protein expression in cell or tissue lysates.
For what are FFPE samples used for?
FFPE human tissue samples are used in medical and scientific research, especially histopathology, as they allow pathologists to diagnose diseases and identify abnormal cellular structures. FFPE samples are also used in immunohistochemistry and in situ hybridization techniques to detect biomarkers, gene expression patterns and to locate proteins. They are also used for genomic studies, transcriptomics analysis and proteomics research. FFPE tissue samples play an important role in drug development, personalized medicine, and biomarker discovery. We offer human tissue samples on our website and gathered more information and FAQs there, feel free to dive deeper in the topic: centralbiohub.de/biospecimens/cancer-samples/ffpe-samples
