Immunohistochemistry information may be found in medical books, studies on biochemistry and physiological sciences. Medical schools have articles and resource materials which contain case studies in which immuunohistochemistry has been used to diagnose patients.
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Paul Hirsch has written: 'Immunochemische Studien' -- subject(s): Immunohistochemistry
There could be multiple reasons why immunohistochemistry staining may work on control tissue but not on experimental tissue, such as differences in antigen expression, tissue processing methods, or antibody specificity. It's important to carefully review and troubleshoot the staining protocol, as well as consider factors like fixation and processing conditions that may affect the staining outcome. Additionally, confirming the presence of the antigen of interest in the experimental tissue using alternative methods can help identify potential issues with the immunohistochemistry staining.
Ethanol hydration in immunohistochemistry is used to rehydrate tissue sections that have been dehydrated during the staining process. This step allows for better penetration of antibodies and reagents into the tissue, improving the overall staining quality and specificity.
BioCare Medical provides high quality immunohistochemistry and molecular pathology products. They have ancillaries, antibodies, and equipment for detection.
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