0
For Mycobacterium you will use the Acid-fast staining technique. There are two different methods of stainging: 1) Ziehl-Neelsen Method and 2) Kinyoun Method.
1) The Ziel-Neelsen method uses a primary stain of Carbol Fuchsin dye that must be steam treated, rinsed with acid alcohol wash, and a secondary stain of Methylene Blue.
2) The Kinyoun Method uses a primary stain of Kinyoun Carbol Fuchsin dye that is not steam treated. An acid alcohol wash is applied and a secondary dye of Brilliant Green. This technique is called "cold staining".
The mycolic acid within the Mycobacterium cell membrane has a high affinity for the Carbol Fuchsin dyes.
What else can I help you with?
Which specific Mycobacterium organism was Ehrlich trying to stain when he developed the acid-fast stain?
Ehrlich was trying to stain Mycobacterium tuberculosis, the bacterium that causes tuberculosis. The acid-fast stain he developed allows for the visualization of the unique cell wall properties of this bacterium, which resists conventional staining methods.
Gram stain and shape of mycobacterium avium?
Mycobacterium avium is a rod-shaped bacterium that typically appears as pink/red on a Gram stain due to its acid-fast characteristics. It is a slow-growing, non-motile bacterium that is commonly found in the environment and can infect humans, particularly those with compromised immune systems.
What is 'Mycobacterium scrofulaceum'?
Mycobacterium scrofulaceum is a species of bacteria belonging to the Mycobacterium genus. It is a non-tuberculous mycobacterium that can cause lymphadenitis in humans, particularly affecting the lymph nodes in the neck. It is part of the Mycobacterium avium complex and is typically resistant to many antibiotics.
Is maneval's stain an acid stain?
Yes, Maneval's stain is an acid-fast stain used in microbiology to detect acid-fast bacteria such as Mycobacterium species. It involves using acid-alcohol to decolorize non-acid-fast bacteria while acid-fast bacteria retain the stain due to their waxy cell wall.
What is carbolfuchsin?
This compound is basic.
Which stain would be the best choice for detecting mycobacterium the bacteria responsible for tuberculosis and leprosy?
ZN stain
Which specific Mycobacterium organism was Ehrlich trying to stain when he developed the acid-fast stain?
Ehrlich was trying to stain Mycobacterium tuberculosis, the bacterium that causes tuberculosis. The acid-fast stain he developed allows for the visualization of the unique cell wall properties of this bacterium, which resists conventional staining methods.
What is fluorochrome staining technique for mycobacterium?
A flourochrome stain is an ultraviolet reactive dye that shines when exposed to UV. By using this stain, acid-fast bacilli (such as mycobacterium) can be easily seen. Flourochrome is used because standard techniques such as Gram stains can result in inconclusive results.
What is 'Mycobacterium scrofulaceum'?
Mycobacterium scrofulaceum is a species of bacteria belonging to the Mycobacterium genus. It is a non-tuberculous mycobacterium that can cause lymphadenitis in humans, particularly affecting the lymph nodes in the neck. It is part of the Mycobacterium avium complex and is typically resistant to many antibiotics.
What bacteria is used in acid-fast staining?
Mycobacterium species, such as Mycobacterium tuberculosis, are the bacteria commonly used in acid-fast staining due to their high lipid content in the cell wall, which makes them resistant to destaining with acid-alcohol solutions. This characteristic allows them to retain the primary stain, carbol fuchsin, and appear "acid-fast" red under the microscope.
Gram stain and shape of mycobacterium avium?
Mycobacterium avium is a rod-shaped bacterium that typically appears as pink/red on a Gram stain due to its acid-fast characteristics. It is a slow-growing, non-motile bacterium that is commonly found in the environment and can infect humans, particularly those with compromised immune systems.
What are the name of cold acid fast stain?
The cold acid-fast stain used for Mycobacterium tuberculosis is called the Kinyoun stain or the Kinyoun cold stain. This staining technique is commonly used to identify acid-fast bacteria that are not easily stained by traditional methods.
Is maneval's stain an acid stain?
Yes, Maneval's stain is an acid-fast stain used in microbiology to detect acid-fast bacteria such as Mycobacterium species. It involves using acid-alcohol to decolorize non-acid-fast bacteria while acid-fast bacteria retain the stain due to their waxy cell wall.
What is carbolfuchsin?
This compound is basic.
Is the mycobacterium smegmatis positive to acid-fast?
Yes, Mycobacterium smegmatis is positive for acid-fast staining due to its thick, waxy cell wall. This characteristic allows it to resist decolorization by acid-alcohol during the staining process, leading to retention of the primary stain (carbol fuchsin) and appearing pink or red under a microscope.
Is mordant used in acid fast stain?
Yes, a mordant is used in the acid-fast stain technique. The mordant used is heat to help drive the primary stain, usually carbol-fuchsin, into acid-fast bacteria, such as Mycobacterium species, which resist decolorization with acid-alcohol.
What are the differences between the Gram stain and acid-fast stain techniques in microbiology?
The Gram stain technique is used to differentiate bacteria into two groups based on their cell wall composition (Gram-positive and Gram-negative), while the acid-fast stain technique is used to detect bacteria that have a waxy cell wall, such as Mycobacterium species.
