0
For Mycobacterium you will use the Acid-fast staining technique. There are two different methods of stainging: 1) Ziehl-Neelsen Method and 2) Kinyoun Method.
1) The Ziel-Neelsen method uses a primary stain of Carbol Fuchsin dye that must be steam treated, rinsed with acid alcohol wash, and a secondary stain of Methylene Blue.
2) The Kinyoun Method uses a primary stain of Kinyoun Carbol Fuchsin dye that is not steam treated. An acid alcohol wash is applied and a secondary dye of Brilliant Green. This technique is called "cold staining".
The mycolic acid within the Mycobacterium cell membrane has a high affinity for the Carbol Fuchsin dyes.
Wiki User
∙ 14y ago
Add your answer:
Earn +20 pts
Gram stain and shape of mycobacterium avium?
Mycobacterium avium is gram-positive and is the shape of bassilous. What I don't understand is why people can't just look this up.... :-/
Is the mycobacterium smegmatis positive to acid-fast?
NO, s. epidermidis does not show up positive in an acid fast stain. The acid fast stain is a differential stain that differentiates cells with mycolic acids in their cells walls with those who do not. cells with mycolic acid are therefore acid fast. s epidermidis does not contain this waxy substance in their cell wall so it doesnt fall into this category.
What is 'Mycobacterium scrofulaceum'?
m.scrofulaceum is a kind of mycobacterum that causes cervical lymphadenitis in kids.
What is the fluorochrome staining technique for mycobacterium?
a fluorescent dye used to stain the cell walls of fungi and bacteria. The organisms then fluoresce when exposed to UV light rays. It is commonly used to visualize acid-fast bacilli (mycobacteria) in specimens. An example is acridine orange staincomsats Pharmacy (sandboy_2008@yahoo.com)
What kind of stain are used to stain plant and animal cells?
Methylene blue stain is used to stain plant and animal cells.
Which stain would be the best choice for detecting mycobacterium the bacteria responsible for tuberculosis and leprosy?
ZN stain
Gram stain and shape of mycobacterium avium?
Mycobacterium avium is gram-positive and is the shape of bassilous. What I don't understand is why people can't just look this up.... :-/
What is fluorochrome staining technique for mycobacterium?
A flourochrome stain is an ultraviolet reactive dye that shines when exposed to UV. By using this stain, acid-fast bacilli (such as mycobacterium) can be easily seen. Flourochrome is used because standard techniques such as Gram stains can result in inconclusive results.
What bacteria is used in acid-fast staining?
It is used for those bacteria which contain fat or lipid layer on their outer wall, and did not stain with grams staining. e.g. Mycobacterium
Is the mycobacterium smegmatis positive to acid-fast?
NO, s. epidermidis does not show up positive in an acid fast stain. The acid fast stain is a differential stain that differentiates cells with mycolic acids in their cells walls with those who do not. cells with mycolic acid are therefore acid fast. s epidermidis does not contain this waxy substance in their cell wall so it doesnt fall into this category.
What is 'Mycobacterium scrofulaceum'?
m.scrofulaceum is a kind of mycobacterum that causes cervical lymphadenitis in kids.
What kind of stain would you use to visualize Sars?
Negative stain
What kind of stain are used to stain plant and animal cells?
Methylene blue stain is used to stain plant and animal cells.
What is the fluorochrome staining technique for mycobacterium?
a fluorescent dye used to stain the cell walls of fungi and bacteria. The organisms then fluoresce when exposed to UV light rays. It is commonly used to visualize acid-fast bacilli (mycobacteria) in specimens. An example is acridine orange staincomsats Pharmacy (sandboy_2008@yahoo.com)
Why was Mycobacterium smegmatis used instead of Mycobacterium tuberculosis?
Mycobacterium smegmatis is a non pathogenic organism so it is used in labs to study about Mycobacterium tuberculosis .
What do Mycoplasma sp and Mycobacteria sp.have in common?
Both stain poorly with the Gram stain.However Mycoplasma has no cell wall while Mycobacterium has a thick cell wall of mycolic acids.
When was Mycobacterium pinnipedii created?
Mycobacterium pinnipedii was created in 2003.
