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A flourochrome stain is an ultraviolet reactive dye that shines when exposed to UV. By using this stain, acid-fast bacilli (such as mycobacterium) can be easily seen. Flourochrome is used because standard techniques such as Gram stains can result in inconclusive results.
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What is the fluorochrome staining technique for mycobacterium?
a fluorescent dye used to stain the cell walls of fungi and bacteria. The organisms then fluoresce when exposed to UV light rays. It is commonly used to visualize acid-fast bacilli (mycobacteria) in specimens. An example is acridine orange staincomsats Pharmacy (sandboy_2008@yahoo.com)
What are examples of acid-fast organisms?
Examples of acid-fast organisms include Mycobacterium tuberculosis, Mycobacterium leprae, and Nocardia species. Acid-fast staining is a technique used to detect these bacteria, as they have a waxy substance in their cell walls that makes them resistant to standard staining methods.
What is the Structure of mycobacterium tuberculosis in carbol fushin staining?
Mycobacterium tuberculosis is an obligate species in the family Mycobacterium and the causative agent of most cases of tuberculosis
What would be an acid-fast bacteria?
Mycobacterium is am example for acid fast bacteria. These bacterias have large amounts of mycolic acids in their cell wall which are impermeable to any other staining technique.
Which staining technique is used to identify the simple stain(s)?
The staining technique used to identify simple stains is called the simple staining technique.
What is the specific diagnostic value of acid-fast stain?
Acid-fast stain is specifically used to detect mycobacteria, such as Mycobacterium tuberculosis, which are resistant to decolorization by acid-alcohol after staining with carbol fuchsin. This staining technique helps in the diagnosis of tuberculosis and other mycobacterial infections.
Where does Mycobacterium tuberculosis hide?
Mycobacterium tuberculosis does not hide; it simply has a coating that makes it hard to detect. This waxy coating on its cell surface makes the cell impervious to gram staining.
How does the technique works of the direct immunofluroscence?
Immunofluorescence is a technique allowing the visualization of a specific protein or antigen in cells or tissue sections by binding a specific antibody chemically conjugated with a fluorescent dye such as fluorescein isothiocyanate (FITC). There are two major types of immunofluorescence staining methods: 1) direct immunofluorescence staining in which the primary antibody is labeled with fluorescence dye, and 2) indirect immunofluorescence staining in which a secondary antibody labeled with fluorochrome is used to recognize a primary antibody. Immunofluorescence staining can be performed on cells fixed on slides and tissue sections. Immunofluorescence stained samples are examined under a fluorescence microscope or confocal microscope
What kind of stain do you stain Mycobacterium with?
For Mycobacterium you will use the Acid-fast staining technique. There are two different methods of stainging: 1) Ziehl-Neelsen Method and 2) Kinyoun Method.1) The Ziel-Neelsen method uses a primary stain of Carbol Fuchsin dye that must be steam treated, rinsed with acid alcohol wash, and a secondary stain of Methylene Blue.2) The Kinyoun Method uses a primary stain of Kinyoun Carbol Fuchsin dye that is not steam treated. An acid alcohol wash is applied and a secondary dye of Brilliant Green. This technique is called "cold staining".The mycolic acid within the Mycobacterium cell membrane has a high affinity for the Carbol Fuchsin dyes.
What are the two common techniques used to visualize endospores?
The two common techniques used to visualize endospores are Schaeffer-Fulton method and the Dorner technique. The Schaeffer-Fulton method involves staining the endospores with malachite green and safranin, while the Dorner technique uses fluorescence microscopy to visualize endospores stained with a fluorochrome dye.
What is the purpose of differential staining?
differential staining is a staining technique used to stain colorless bacteria against a dark background.
Is crystal violet considered a primary stain in the Gram staining technique?
Yes, crystal violet is considered a primary stain in the Gram staining technique.
