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a fluorescent dye used to stain the cell walls of fungi and bacteria. The organisms then fluoresce when exposed to UV light rays. It is commonly used to visualize acid-fast bacilli (mycobacteria) in specimens. An example is acridine orange stain
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∙ 12y ago
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∙ 9y agoA flourochrome stain is an ultraviolet reactive dye that shines when exposed to UV. By using this stain, acid-fast bacilli (such as mycobacterium) can be easily seen. Flourochrome is used because standard techniques such as Gram stains can result in inconclusive results.
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What kind of stain do you stain Mycobacterium with?
For Mycobacterium you will use the Acid-fast staining technique. There are two different methods of stainging: 1) Ziehl-Neelsen Method and 2) Kinyoun Method.1) The Ziel-Neelsen method uses a primary stain of Carbol Fuchsin dye that must be steam treated, rinsed with acid alcohol wash, and a secondary stain of Methylene Blue.2) The Kinyoun Method uses a primary stain of Kinyoun Carbol Fuchsin dye that is not steam treated. An acid alcohol wash is applied and a secondary dye of Brilliant Green. This technique is called "cold staining".The mycolic acid within the Mycobacterium cell membrane has a high affinity for the Carbol Fuchsin dyes.
Which technique helped scientists in the 1800 to identify cell organelles?
Staining
What is the different staining technique in virology?
1- What_is_the_different_staining_technique_in_virology2- What are the diffrent stain in micro for virus ?
What makes Mycobacterium resistant to staining?
It has a peptidoglycan layer filled with mycolic acids. The acids make the cell wall waxy and impenetrable to stains. They are classified with gram positive cells because of cell wall thickness and genetic similarities.
What is a acid fast disease?
Some diseases are leprosy ( M. leprae), tuberculosis (M. tuberculosis), and nocardiosis ( N. brasiliensis, N. asteroides). The acid fast stain is important in identifying bacteria in the genus Mycobacterium and Nocardia.M. tuberculosis
What is fluorochrome staining technique for mycobacterium?
A flourochrome stain is an ultraviolet reactive dye that shines when exposed to UV. By using this stain, acid-fast bacilli (such as mycobacterium) can be easily seen. Flourochrome is used because standard techniques such as Gram stains can result in inconclusive results.
What is the Structure of mycobacterium tuberculosis in carbol fushin staining?
Mycobacterium tuberculosis is an obligate species in the family Mycobacterium and the causative agent of most cases of tuberculosis
What would be an acid-fast bacteria?
Mycobacterium is am example for acid fast bacteria. These bacterias have large amounts of mycolic acids in their cell wall which are impermeable to any other staining technique.
How does the technique works of the direct immunofluroscence?
Immunofluorescence is a technique allowing the visualization of a specific protein or antigen in cells or tissue sections by binding a specific antibody chemically conjugated with a fluorescent dye such as fluorescein isothiocyanate (FITC). There are two major types of immunofluorescence staining methods: 1) direct immunofluorescence staining in which the primary antibody is labeled with fluorescence dye, and 2) indirect immunofluorescence staining in which a secondary antibody labeled with fluorochrome is used to recognize a primary antibody. Immunofluorescence staining can be performed on cells fixed on slides and tissue sections. Immunofluorescence stained samples are examined under a fluorescence microscope or confocal microscope
Where does Mycobacterium tuberculosis hide?
Mycobacterium tuberculosis does not hide; it simply has a coating that makes it hard to detect. This waxy coating on its cell surface makes the cell impervious to gram staining.
What is the purpose of differential staining?
differential staining is a staining technique used to stain colorless bacteria against a dark background.
What kind of stain do you stain Mycobacterium with?
For Mycobacterium you will use the Acid-fast staining technique. There are two different methods of stainging: 1) Ziehl-Neelsen Method and 2) Kinyoun Method.1) The Ziel-Neelsen method uses a primary stain of Carbol Fuchsin dye that must be steam treated, rinsed with acid alcohol wash, and a secondary stain of Methylene Blue.2) The Kinyoun Method uses a primary stain of Kinyoun Carbol Fuchsin dye that is not steam treated. An acid alcohol wash is applied and a secondary dye of Brilliant Green. This technique is called "cold staining".The mycolic acid within the Mycobacterium cell membrane has a high affinity for the Carbol Fuchsin dyes.
What bacteria is used in acid-fast staining?
It is used for those bacteria which contain fat or lipid layer on their outer wall, and did not stain with grams staining. e.g. Mycobacterium
What are examples of acid-fast organisms?
Two acid-fast bacteria that are pathogenic are:1. Mycobacterium tuberculosis2. Mycobacterium avium- Microbioman
What is mordant in z-n staining technique?
Phenol
What types of organisms are subjected to Negative Staining Technique?
Bacteria .
The technique used to detect corneal abrasions is?
Fluorescein staining
