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What is the purpose of the heat during the acid fast staining procedure?
The heat is used to drive the primary stain, carbol fuchsin, into the waxy cell wall of acid-fast bacteria. This allows the stain to penetrate the mycolic acid in the cell wall, making the bacteria resistant to decolorization with acid-alcohol.
What is the counter or secondary stain used in the acid-fast stain technique?
The counter or secondary stain used in the acid-fast stain technique is methylene blue.
Is maneval's stain an acid stain?
Yes, Maneval's stain is an acid-fast stain used in microbiology to detect acid-fast bacteria such as Mycobacterium species. It involves using acid-alcohol to decolorize non-acid-fast bacteria while acid-fast bacteria retain the stain due to their waxy cell wall.
Why is the counter stain for gram stain procedure differ from the counter stain used for acid fast procedure?
The counter stain used in the Gram stain procedure is typically safranin or basic fuchsin, which stains Gram-negative bacteria pink or red. In the acid-fast stain procedure, the counter stain used is typically methylene blue or brilliant green, which stains non-acid-fast bacteria blue or green, allowing acid-fast bacteria to retain the primary stain color (carbolfuchsin).
Why isn't the Gram stain used on acid-fast bacteria?
The Gram stain is not effective on acid-fast bacteria because their cell walls have a waxy layer that prevents the stain from penetrating.
What is mordant in z-n staining technique?
In Ziehl-Neelsen staining technique, a mordant such as heat or steam is used to enhance the binding of the primary stain (carbolfuchsin) to the acid-fast bacteria. The mordant helps the stain penetrate the waxy cell walls of acid-fast bacteria, improving the visualization of these organisms under the microscope.
What is the purpose of the heat during the acid fast staining procedure?
The heat is used to drive the primary stain, carbol fuchsin, into the waxy cell wall of acid-fast bacteria. This allows the stain to penetrate the mycolic acid in the cell wall, making the bacteria resistant to decolorization with acid-alcohol.
What mordant is used in spore staining?
Heat is the mordant used in the spore stain, it fixes the primary stain.
What is the counter or secondary stain used in the acid-fast stain technique?
The counter or secondary stain used in the acid-fast stain technique is methylene blue.
What are the name of cold acid fast stain?
The cold acid-fast stain used for Mycobacterium tuberculosis is called the Kinyoun stain or the Kinyoun cold stain. This staining technique is commonly used to identify acid-fast bacteria that are not easily stained by traditional methods.
Is maneval's stain an acid stain?
Yes, Maneval's stain is an acid-fast stain used in microbiology to detect acid-fast bacteria such as Mycobacterium species. It involves using acid-alcohol to decolorize non-acid-fast bacteria while acid-fast bacteria retain the stain due to their waxy cell wall.
Why is the counter stain for gram stain procedure differ from the counter stain used for acid fast procedure?
The counter stain used in the Gram stain procedure is typically safranin or basic fuchsin, which stains Gram-negative bacteria pink or red. In the acid-fast stain procedure, the counter stain used is typically methylene blue or brilliant green, which stains non-acid-fast bacteria blue or green, allowing acid-fast bacteria to retain the primary stain color (carbolfuchsin).
Are endospores acid fast?
Yes, endospores are acid-fast because their thick walls are resistant to most stains and dyes, including the acid-fast stain used in microbiology to detect mycobacteria like Mycobacterium tuberculosis. This resistance allows endospores to retain the stain even after decolorization with acid-alcohol.
Why isn't the Gram stain used on acid-fast bacteria?
The Gram stain is not effective on acid-fast bacteria because their cell walls have a waxy layer that prevents the stain from penetrating.
What is oxalic acid used for?
It is a weak acid mainly used for TITRATION
What type of dye is used to stain the specimen when the acid-fast stain and the gram stain are used?
Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
Other name for ziehl neelsen?
The Ziehl-Neelsen stain, also known as the acid fast stain, was first described by two German doctors, Franz Ziehl, a bacteriologist and Friedrich Neelsen a pathologist. It is a special bacteriological stain used to identify acid-fast organisms, mainly Mycobacteria.
