0
What is the purpose of the heat during the acid fast staining procedure?
Wiki User
∙ 13y ago
It acts as the mordant to soften the mycolic acid so that the stain can penetrate the cell.
Wiki User
∙ 13y ago
Add your answer:
Earn +20 pts
What staining procedure will be used to observe bacteria in the sputum sample?
acid fast staining or Ziel Neelson staining for observing Mycobacteria tuberculosis or Koch bacilli from sputum sample.
What are the consequences of Ziehl-Neelsen staining?
The Ziehl-Neelsen stain is also known as the acid-fast stain. It contains sulfuric acid, and is used to identify acid-fast bacteria, or bacteria resistant to decolorization by acids from staining.
Why do you use acid fast stain?
Because it is a type of stain that uses contrasting (in this case the presence or absence of a stain) color to determine what type or classification the organism is. Differential = determining between different groups; you are staining to differentiate one group from another by classifying the organisms' ability to quickly resist decolorization by acids.
What makes a microorganism non-acid-fast?
The low lipid content in the cells ----------------------------------------------- The above answer is not wrong. I'm just giving adding more information to it. Acid-fast staining is due to the high lipid content (mycolic acid) in the cell wall. Cells that do not have these mycolic acids do not absorb the carbolfuchsin. Microorganisms that have taken up the carbolfuchsin are not easily decolorized by the acid-alcohol step in the preparation procedure.
What so endospore stain have in common with the acid-fast stain?
One thing that endospore stains have in common with the acid fast stain is that heat primary stain penetration. Another thing that endospore stains have in common with acid fast stains are counterstain.
What is the purpose of the heat during the staining procedures?
It acts as the mordant to soften the mycolic acid so that the stain can penetrate the cell.
What staining procedure will be used to observe bacteria in the sputum sample?
acid fast staining or Ziel Neelson staining for observing Mycobacteria tuberculosis or Koch bacilli from sputum sample.
Why acid alcohol is used instead of ethyl alcohol in acid fast staining?
Acid alcohol destains non-acid fast bacteria but not Mycobacteria, which are resistant to the procedure due to the presence of mycolic acid. In the Ziehl Neelsen procedure, Mycobacteria remain red from the carbolfuchsin primary stain after destaining and non-acid fast bacteria (or tissue) which lose the primary stain during the destaining procedure are counterstained blue by methylene blue.
How do you decolourise schiffs reagent?
With periodic acid during the steps of staining slides.
What is the practical importance in a medical lab of the acid-fast stain?
By this technique, we can diffentiate the acid fast and non acid- fast bacteria. The non acid-fast bacteria are M.tuberculosis and N.asteriodes. They are the causative agents for tuberculosis and nocardiosis respectively. The acid fast staining or the Ziel- Nielsen's staining is the only procedure to find out the above mentioned pathogens.
Why steam heating is done in acid fast staining and not heat fixing?
Actually, both methods are used during the staining procedure (steam & heat fix). Initially, the organism is heat fixed to the slide to prevent the organism from being washed off during subsequent steps. Later in the procedure, the slide with the heat fixed organism is steamed to make the cell wall a little more penetrable - allowing the stain to enter the cell wall.
What is staining in a pool?
Acid
Can the acidity due to acetic acid be found out by the titration procedure?
N cannot be found by the titration procedure because the acetic acid is very weak acid and cannot be appear completely during the tit ration procedure
Can the acidity due to acetic be found out by the titration procedure?
N cannot be found by the titration procedure because the acetic acid is very weak acid and cannot be appear completely during the tit ration procedure
Why is mycobacterium tuberculosis considered acid fast?
Saprophytic mycobacteria are acid fast and do not cause serious disease.
What is the purpose of Trichloroacetic acid in DNA extraction?
Trichloroacetic acid is used for precipitation of the DNA during its extraction.
What are the consequences of Ziehl-Neelsen staining?
The Ziehl-Neelsen stain is also known as the acid-fast stain. It contains sulfuric acid, and is used to identify acid-fast bacteria, or bacteria resistant to decolorization by acids from staining.
