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What are the acid-fast stain results for Bacillus subtilis?
Bacillus subtilis is a Gram-positive bacterium and does not typically show acid-fast staining results. This means that it does not retain the stain when subjected to the acid-fast staining procedure commonly used to detect mycobacteria.
What staining procedure will be used to observe bacteria in the sputum sample?
A Gram stain is commonly used to observe bacteria in a sputum sample. This staining procedure helps visualize the bacterial cell wall structure and arrangement, aiding in the identification of different bacterial species.
Is the acid fast staining result positive or negative for the sample?
The acid-fast staining result for the sample is positive.
What does acid base bacilli?
Acid-fast bacilli are a group of bacteria that resist decolorization by acids during laboratory staining procedures. These bacilli include species such as Mycobacterium tuberculosis, which causes tuberculosis. Acid-fast staining helps in their identification due to their unique cell wall composition.
What makes a microorganism non-acid-fast?
The low lipid content in the cells ----------------------------------------------- The above answer is not wrong. I'm just giving adding more information to it. Acid-fast staining is due to the high lipid content (mycolic acid) in the cell wall. Cells that do not have these mycolic acids do not absorb the carbolfuchsin. Microorganisms that have taken up the carbolfuchsin are not easily decolorized by the acid-alcohol step in the preparation procedure.
What is the purpose of the heat during the staining procedures?
It acts as the mordant to soften the mycolic acid so that the stain can penetrate the cell.
What are the acid-fast stain results for Bacillus subtilis?
Bacillus subtilis is a Gram-positive bacterium and does not typically show acid-fast staining results. This means that it does not retain the stain when subjected to the acid-fast staining procedure commonly used to detect mycobacteria.
What staining procedure will be used to observe bacteria in the sputum sample?
A Gram stain is commonly used to observe bacteria in a sputum sample. This staining procedure helps visualize the bacterial cell wall structure and arrangement, aiding in the identification of different bacterial species.
Is M. tuberculosis acid fast or non-acid fast?
Mycobacterium tuberculosis is an acid fast bacterium. It has a high concentration of mycolic acids in the plasma membrane which prevent its staining by typical Gram stain methods. It must be stained with a procedure containing an acid decolorizing step to best visualize it under the microscope (Ziehl Nielson or Kinyon Methods). It resists decolorization with the acid, which is where the term "Acid Fast" comes from....
What is staining in a pool?
Acid
Is the acid fast staining result positive or negative for the sample?
The acid-fast staining result for the sample is positive.
Why acid alcohol is used instead of ethyl alcohol in acid fast staining?
Acid alcohol destains non-acid fast bacteria but not Mycobacteria, which are resistant to the procedure due to the presence of mycolic acid. In the Ziehl Neelsen procedure, Mycobacteria remain red from the carbolfuchsin primary stain after destaining and non-acid fast bacteria (or tissue) which lose the primary stain during the destaining procedure are counterstained blue by methylene blue.
Can the acidity due to acetic acid be found out by the titration procedure?
N cannot be found by the titration procedure because the acetic acid is very weak acid and cannot be appear completely during the tit ration procedure
Why steam heating is done in acid fast staining and not heat fixing?
Actually, both methods are used during the staining procedure (steam & heat fix). Initially, the organism is heat fixed to the slide to prevent the organism from being washed off during subsequent steps. Later in the procedure, the slide with the heat fixed organism is steamed to make the cell wall a little more penetrable - allowing the stain to enter the cell wall.
Can the acidity due to acetic be found out by the titration procedure?
N cannot be found by the titration procedure because the acetic acid is very weak acid and cannot be appear completely during the tit ration procedure
What bacteria which are weak acid fast?
Mycobacterium avium complex (MAC) bacteria are weakly acid-fast, meaning they retain some of the carbol fuchsin stain when decolorized with acid-alcohol during acid-fast staining. This makes them appear weakly positive in acid-fast staining techniques.
What does acid base bacilli?
Acid-fast bacilli are a group of bacteria that resist decolorization by acids during laboratory staining procedures. These bacilli include species such as Mycobacterium tuberculosis, which causes tuberculosis. Acid-fast staining helps in their identification due to their unique cell wall composition.
