Bacillus subtilis is a Gram-positive bacterium and does not typically show acid-fast staining results. This means that it does not retain the stain when subjected to the acid-fast staining procedure commonly used to detect mycobacteria.
gram positive Exactly. When doing a gram stain on B. subtilis, this bacterium resists decolorization (keping the first stain and NOT taking on the color of the secondary stain). Therefore, this bacterium is gram (+).
B. subtilis is indeed a Gram positive rod.However, there is confusion as to whether it is an obligate aerobe or a facultative anaerobe. Although classed as an obligate aerobe, it has been shown to grow under strict anaerobic conditions.
Bacillus cells stained with malachite green and safranin will appear red under the microscope due to the counterstaining with safranin. Malachite green primarily stains the spores of Bacillus while safranin stains the rest of the cell, resulting in red-stained vegetative cells and green-stained spores.
The Kirby-Bauer antibiotic test is a general test that determines if an organism is resistant or susceptible to different antibiotics. Many different types of antibiotics can be used but some of the common ones are penicillin, tetracycline, chloramphenicol, and streptomycin.
The age of the culture used for a spore stain can impact the results by affecting the viability and sporulation of the organism. A young culture with actively growing cells is more likely to produce good spore stain results, while an older culture with decreased viability and sporulation may lead to unreliable staining outcomes. It is generally recommended to use a fresh culture for spore staining to obtain accurate and reliable results.
gram positive Exactly. When doing a gram stain on B. subtilis, this bacterium resists decolorization (keping the first stain and NOT taking on the color of the secondary stain). Therefore, this bacterium is gram (+).
Bacillus subtilis typically forms large, dry, irregular colonies with a matte appearance on MYP agar, while Staphylococcus aureus usually forms small, smooth, shiny colonies. Additionally, B. subtilis is often motile and may exhibit rhizoid growth patterns, while S. aureus is non-motile. A Gram stain can also be useful, as B. subtilis is Gram-positive and S. aureus is Gram-positive.
B. subtilis is indeed a Gram positive rod.However, there is confusion as to whether it is an obligate aerobe or a facultative anaerobe. Although classed as an obligate aerobe, it has been shown to grow under strict anaerobic conditions.
The Gram stain for anthrax shows large, Gram-positive bacilli that appear as long chains of cells. Anthrax bacteria stain blue or purple because of their thick peptidoglycan cell wall, which retains the crystal violet dye used in the Gram staining procedure.
Methylene blue stains everything blue.
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Yes, a pre-stain conditioner is recommended for optimal results when staining wood as it helps to even out the absorption of the stain and prevent blotchiness.
Yes, you can apply wood conditioner over stain for optimal results. Wood conditioner helps to even out the absorption of stain, resulting in a more uniform finish.
For optimal results, it is recommended to apply 2-3 coats of stain on pine wood.
For the best results on oil-based stain, use a polyurethane with an oil-based formula.
You should stain a new deck after it has been built and allowed to weather for at least 3-6 months. This allows the wood to dry out and be ready to absorb the stain properly, resulting in the best results.
It is recommended to wait at least 24 hours between applying stain and polyurethane to allow the stain to fully dry and set, ensuring the best results for your project.