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B. subtilis is indeed a Gram positive rod.

However, there is confusion as to whether it is an obligate aerobe or a facultative anaerobe. Although classed as an obligate aerobe, it has been shown to grow under strict anaerobic conditions.

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14y ago
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13y ago

In an acid-fast stain, positive acid-fast bacterium will appear red because they possess a lipid mycolic acid on their membrane and will retain heated carbolfuchsin. Negative acid-fast bacterium will appear blue (stained by methylene blue). Myobacterium and nocardia are negative acid-fast bacterium. All the rest of bacterium (i.e. bacillus) are acid-fast positve.

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Whoever gave that answer above should not be posting anything again. That answer is completely wrong (except for the explanation). Mycobacterium and Nocardia are acid-fast positive. I don't know what all the bacteria are that give off a negative, but Bacillus subtilis and Corynebacterium are acid-fast negative.

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11y ago

Bacillus subtilis retains crystal violate dye in both the simple stain and the gram stain. Thus, the morphology of the organism is the same after both procedures. Regardless of simple stain or gram stain, if you know you're dealing with B. subtilis, you'll see purple rods that are sort of clustered together. Remember that the simple stain is only really used to increase contrast between the organism at hand and the field of view in the microscope.

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14y ago

it is Gram positive

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11y ago

It is blue and rod shaped.

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8y ago

DNA

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11y ago

It's Gram positive

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Q: What is the bacteria mophology for bacillus subtilis by simple stain?
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