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ACID FAST Os Contains waxlike lipoidal material affecting staining quality. Carbolfuchsin is primary stain. Acid fast organisms resist decolorization with acid alcohol. After decolorization, methyelene blue is added to organisms to counterstain any material that is not acid fast. It is used for examination of Mycobacterium.
The principle of staining is depend on the resistance of this type of bacteria to decolarization by acid alcohol, because the cell wall contain waxy material (mycolic acid) which prevent the removal of carbol fuchsin from the cell.
Staining Procedure
1. Flood the slide with strong carbol fuchsin and heat until steam rises (but do not boil).
2. After 3-4 min apply more heat until rises again; do not let the stain dry on the slide.
3. About 5-7 min after the first application of heat washes the slide thoroughly under running water.
4. Decolourize in acid-alcohol until all traces of red have disappeared from the film. Decolourization should not be attempted in one stage; there should be intermittent washings in water and re-application of acid-alcohol.
5. Wash well with water and counter stain with Methylene blue for 1 min.
6. Wash and stand end to drain.
7. Acid-fast organisms are red, other organisms are blue.
What else can I help you with?
What is the action of iodine in grams stain?
Iodine is used to bind the Crystal Violet to the Gram Positive microbes.
Can a Gram stain a prion?
Protists are often stained using a silver stain, not a Gram stain.
Wrights stain or other stains?
Wright's stain is a Romanowsky stain used in medical laboratories to differentiate blood cells for microscopy. Other stains used for blood cell differentiation include Giemsa stain, Leishman stain, and Hematoxylin and eosin stain. Each stain has specific applications and staining properties.
How do surfactants work in stain removers?
Surfactants in stain removers work by reducing the surface tension of water, allowing it to more easily penetrate and lift stains from fabric. They help to break down the bonds between the stain molecules and the fabric, making it easier to remove the stain during the washing process.
I am wondering Hematoxylin stain and Harris Hematoxylin stain are the same thing or not ie. Papanicolaou stain includes Harris Hematoxylin stain and H and E stain includes Hematoxylin stain thanks?
Hematoxylin is only the drastic substance that these solutions contain. For histology, the two most commonly used are Mayer's Hematoxylin and Harris' Hematoxylin. They both contain water, hematoxylin and various salts.
How could you describe a stain instead of saying a big stain what could you say?
a huge stain. a jumbo stain. a large stain.
What is commonly used in the Shout Triple Three Action stain remover?
The Shout Triple Action stain remover commonly uses a combination of enzymes, surfactants, and cleaning agents to break down and remove tough stains from clothing and fabrics.
What is the action of iodine in grams stain?
Iodine is used to bind the Crystal Violet to the Gram Positive microbes.
How do you remove coffee stain is Vanish stain remover good?
Yes, Vanish stain remover is great to get rid of coffee stains. You need to dissolve one scoop of Vanish Oxi Action Powder in 4L of warm water and soak the stain. For coloured fabrics, soak for max 1 hour and whites for max 6 hours. Wash as usual.
What are the action of solvent in stain removal?
Solvents act by breaking down and dissolving the components of a stain, making it easier to remove from a surface. They help to weaken the bond between the stain and the material it has attached to, allowing for effective removal. Solvents are particularly useful for removing oil-based stains that are not water-soluble.
How would you describe the gram stain?
Describe the Gram stain technique and the effect on Gram-positive and Gram-negative bacteria after each step. Be very specific about what is happening at each step and why it happens. (hint: be sure to fully explain your answer and not just list the steps)
What is the best carpet stain remover?
According to Good Housekeeping, the best carpet stain removers are: Hoover Platinum Collection Instant Bissell OxyPro Weiman Carpet Cleaner RealSimple.com recommends: Resolve Triple Action Spot Carpet Cleaner Carpet stain removers work the best if they are used on the stain as soon as possible. The type of carpet stain remover that works best may also depend on the type of stain you are trying to remove. There are also many home made carpet cleaners which can be used on specific types of stains.
What does a negative result for a spore stain indicate about the organism?
A negative result for a spore stain indicates that the organism does not form endospores. Endospore formation is a survival mechanism for some bacteria to withstand harsh environments, so a negative result suggests that the organism may be more susceptible to environmental stressors.
What is the current theory about the mechanism of the gram stain?
The gram stain is a basic differential stain used to determine if a bacterial cell is gram positive or negative. Gram positive cells have a thick peptidoglycan layer that will trap the crystal violet iodine crystalls and apear purple. Gram negative cells only have a thin peptidoglycan layer that allows the crystals to diffuse out of the cell and will only be seen with the application of a counterstain, such as safranin which turns the cells pink.
Is blood or egg an organic stain or protein stain?
its a protein stain
Why is a stain called a stain?
It's simple, because it's a stain.
Is acid fast stain a type of differential stain?
Yes, acid-fast stain is a type of differential stain.
