0
What else can I help you with?
What is the mordant in a gram stain?
Gram's iodine stain is applied after the culture is stained with the primary stain. It acts as a mordant, fixing the primary stain to the cell wall while lending no additional colour to the cell (i.e. the mordant itself is not a stain). The mordant is only able to fix the stain to Gram-positive bacteria because of the characteristic thick, peptidoglycan coat that they possess. Because the mordant is not able to fix the stain to Gram-negative bacteria (who's coat have a different composition), the crystal violet stain will wash away from Gram-negative bacteria when the decolourizing agent is added.
What is the difference between Iodine bought in a pharmacy and the Iodine stain solution?
The iodine stain solution you're referring to might be Lugol's iodine. This is iodine and potassium iodide in water. The product available in a pharmacy is tincture of iodine which is iodine and potassium iodide in ethanol and water. Please see the links.
What is the secondary stain in gram stain procedure?
It is crystal violet & stains all cells purple.
Why use iodine when preparing slide for viewing?
one disadvantage of using iodine to stain cells is that iodine will kill living cells.
What stain is used amyloplasts?
Iodine stain is commonly used to detect amyloplasts due to their ability to stain starch granules purple-black.
How do you prepare 0.1M IODINE?
2grm iodine and 4grm KI add to 1000ml of water
Which stain you should use to stain onion peel?
To stain onion peel cells, you should use a stain called iodine. Iodine is commonly used to stain cells because it binds to starches and glycogen in the cells, making the cells more visible under a microscope.
How many grams of iodine dissolved in water for 0.02 n iodine?
To calculate the grams of iodine dissolved in water for 0.02 N iodine, you need the molar mass of iodine, which is approximately 254 g/mol. With this information, you can use the formula: Grams = Normality (N) * Equivalent weight. Therefore, for 0.02 N iodine: Grams = 0.02 * 254 = 5.08 grams of iodine.
What is the difference between Iodine bought in a pharmacy and the Iodine stain solution?
The iodine stain solution you're referring to might be Lugol's iodine. This is iodine and potassium iodide in water. The product available in a pharmacy is tincture of iodine which is iodine and potassium iodide in ethanol and water. Please see the links.
What is the mordant in a gram stain?
Gram's iodine stain is applied after the culture is stained with the primary stain. It acts as a mordant, fixing the primary stain to the cell wall while lending no additional colour to the cell (i.e. the mordant itself is not a stain). The mordant is only able to fix the stain to Gram-positive bacteria because of the characteristic thick, peptidoglycan coat that they possess. Because the mordant is not able to fix the stain to Gram-negative bacteria (who's coat have a different composition), the crystal violet stain will wash away from Gram-negative bacteria when the decolourizing agent is added.
What is the secondary stain in gram stain procedure?
It is crystal violet & stains all cells purple.
What does lugol's iodine stain do to the activity of the cell?
I just did a lab in biology called "How Plant and Animal Cells Differ" and in the packet it had that question. The answer is: Lugol's iodine stain stops the activity of the cell. It kills whatever specimen it is staining.
Why nonstaining iodine not stain skin?
Nonstaining iodine is a type of iodine solution that does not contain iodine in a free form that can bind to skin proteins, which is why it does not stain the skin. This means that it is less likely to leave a visible mark on the skin compared to traditional iodine solutions.
Given that zinc reacts with iodine in the fixed mass ratio of 1 gram of zinc to 3.88 grams of iodine how many grams of iodine are required to completely react with 4.2 grams of zinc?
For every 1 gram of zinc, 3.88 grams of iodine are required to react. So in this case, with 4.2 grams of zinc, the amount of iodine needed would be (4.2 grams zinc) * (3.88 grams iodine / 1 gram zinc) = 16.296 grams of iodine.
How many moles are in 222.6 grams of iodine?
222,6 grams of iodine is equivalent to 1,754 moles.
What is the importance of iodine after primary stain?
Iodine is used after the primary stain in the Gram stain procedure to form a complex with the crystal violet dye, which helps to stabilize the dye within the bacterial cell wall. This step enhances the retention of the primary stain in Gram-positive bacteria.
What is the difference between lugols iodine and grams iodine?
Lugol's iodine is a mixture of elemental iodine and potassium iodide, commonly used as a disinfectant and as a supplement to support thyroid function. Gram's iodine, on the other hand, is a stain used in microbiology to differentiate between gram-positive and gram-negative bacteria based on their cell wall composition. The main difference lies in their applications and compositions.
