I just did a lab in Biology called "How Plant and Animal Cells Differ" and in the packet it had that question.
The answer is:
Lugol's iodine stain stops the activity of the cell. It kills whatever specimen it is staining.
lugol's iodine stain stops the activity of the cell.
An iodine stain, specifically Lugol's iodine stain, stops the activity of the cell. It is a vital stain which means that it kills whatever specimen it is staining.
They kill the cell
It stops the activity within the cell.
Iodine work as a modrant which helps in fixing the stain properly by making a compled compoun of stain and cell wall.
Iodine is used in practical experiments such as the onion cell experiment. Iodine will help make the cells visible. Iodine, should it be spilt on skin, can stain skin and will take quite some time to wash off.
As per the exact chemistry, I am unsure, but basically the iodine acts to "set in" the stain by reacting the dye (crystal violet) from the previous step with the peptidoglycan layer, forming a layer which is now insoluble in the following step where the layer is washed with EtOH. If the cell is Gram (-), the iodine doesn't do much and the dye is washed away.
Iodine binds to starch and would be primarily used to stain plant cells. The organelles that would be stained darkest would be the nucleus, amyloplast, which store starches, and chloroplasts which produce it.
the endoSPERM searches for a egg cell and and forms a zygote around the process of iodine solution. WILL YOU EVER BELIEVE THIS DUDE?
Iodine work as a modrant which helps in fixing the stain properly by making a compled compoun of stain and cell wall.
It stops the activity within the cell.
A simple stain like iodine can make cell parts show up that would otherwise be nearly invisible since they are colorless.A simple stain like iodine will reveal a cell's morphology.
Iodine is used to stain the cell. It makes each component of the cell more visible, especially the nucleus.
Iodine stains starch contained in cells. Iodine is also used to distinguish between Gram negative and Gram positive bacteria. The Gram stain contains iodine.
we can use feulgen's method ,in which malachite green stain is used to stain the nucleus
iodine indicates polysaccharides, therefore plant cells can be stained with iodine, staining the chloroplasts- composed of starch(a polysaccharide), and the cell wall- composed of cellulose ( a polysaccharide)
Gram's iodine is called a mordant because it fixes the primary stain to the cell wall and adds any color.
Gram's iodine is called a mordant because it fixes the primary stain to the cell wall and adds any color.
Iodine is an important "marker element" in biology. It is used to trace certain substances such as DNA or RNA during analysis of nucleic acids in a cell. When observing a cell, iodine is used to mark the DNA or RNA in the nucleus and mitochondrion of a cell for easy viewing. So in a way, iodine is a fluorescent organelle highlighter or tag.
Yes, you might just have to stain the cell with iodine. The starch in the cell might block viewage of the nucleus still. It's there though.
Gram's iodine stain is applied after the culture is stained with the primary stain. It acts as a mordant, fixing the primary stain to the cell wall while lending no additional colour to the cell (i.e. the mordant itself is not a stain). The mordant is only able to fix the stain to Gram-positive bacteria because of the characteristic thick, peptidoglycan coat that they possess. Because the mordant is not able to fix the stain to Gram-negative bacteria (who's coat have a different composition), the crystal violet stain will wash away from Gram-negative bacteria when the decolourizing agent is added.