0
What else can I help you with?
What does lugol's iodine stain do to the activity of the cell?
I just did a lab in biology called "How Plant and Animal Cells Differ" and in the packet it had that question. The answer is: Lugol's iodine stain stops the activity of the cell. It kills whatever specimen it is staining.
How do you remove iodine stain from paper?
To remove iodine stains from paper, you can try using a mixture of water and hydrogen peroxide or a diluted solution of bleach. Dab the stained area with the solution using a cloth or cotton ball, then gently blot and rinse with water. Be careful not to oversaturate the paper to avoid damaging it further.
Does iodine make your teeth shine?
Iodine does not make your teeth shine. In fact, iodine can stain your teeth and cause discoloration if used in excessive amounts. It is not recommended to use iodine for dental purposes.
How do you prepare 0.1M IODINE?
2grm iodine and 4grm KI add to 1000ml of water
Why iodine is not directly employed as the primary standard?
Iodine is not directly employed as a primary standard because it is less stable in solution compared to other primary standards like potassium dichromate or potassium permanganate. Additionally, iodine is sensitive to light and air, leading to potential degradation and affecting its accuracy as a primary standard.
What is the secondary stain in gram stain procedure?
It is crystal violet & stains all cells purple.
Why gram's iodine called a mordant?
Gram's iodine is called a mordant because it fixes the primary stain to the cell wall and adds any color.
What is the mordant in a gram stain?
Gram's iodine stain is applied after the culture is stained with the primary stain. It acts as a mordant, fixing the primary stain to the cell wall while lending no additional colour to the cell (i.e. the mordant itself is not a stain). The mordant is only able to fix the stain to Gram-positive bacteria because of the characteristic thick, peptidoglycan coat that they possess. Because the mordant is not able to fix the stain to Gram-negative bacteria (who's coat have a different composition), the crystal violet stain will wash away from Gram-negative bacteria when the decolourizing agent is added.
Which stain you should use to stain onion peel?
To stain onion peel cells, you should use a stain called iodine. Iodine is commonly used to stain cells because it binds to starches and glycogen in the cells, making the cells more visible under a microscope.
What is the difference between Iodine bought in a pharmacy and the Iodine stain solution?
The iodine stain solution you're referring to might be Lugol's iodine. This is iodine and potassium iodide in water. The product available in a pharmacy is tincture of iodine which is iodine and potassium iodide in ethanol and water. Please see the links.
What does lugol's iodine stain do to the activity of the cell?
I just did a lab in biology called "How Plant and Animal Cells Differ" and in the packet it had that question. The answer is: Lugol's iodine stain stops the activity of the cell. It kills whatever specimen it is staining.
Why nonstaining iodine not stain skin?
Nonstaining iodine is a type of iodine solution that does not contain iodine in a free form that can bind to skin proteins, which is why it does not stain the skin. This means that it is less likely to leave a visible mark on the skin compared to traditional iodine solutions.
Can grams iodine be used before primary stain?
Probably not. The CVI complex formed between the iodine and crystal violet will be trapped in the lipid layer and not the peptidoglycan layer, so you would probably end up over decolorizing. This would cause a lot of false gram negatives. Probably not. The CVI complex formed between the iodine and crystal violet will be trapped in the lipid layer and not the peptidoglycan layer, so you would probably end up over decolorizing. This would cause a lot of false gram negatives.
If iodine step was amitted how would gram negative cells appear?
Iodine work as modrant to crystal violet if the iodine step will be emitted then the crystal violet will not be able to show its effect and all cells will appear pink when counter stain with saffranin
What is the function of iodine solution in the gram stain?
It allows the primary stain crystal violet to remain in the cell instead of being washed out. Due to the larger size of the crystal violet molecule, when the ethanol is applied (the decolorizer) the stain will not be washed out of the Gram (+) positive cells.
Why use iodine when preparing slide for viewing?
one disadvantage of using iodine to stain cells is that iodine will kill living cells.
What is the value of simple stain?
A simple stain like iodine can make cell parts show up that would otherwise be nearly invisible since they are colorless.A simple stain like iodine will reveal a cell's morphology.
