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Probably not. The CVI complex formed between the iodine and crystal violet will be trapped in the lipid layer and not the peptidoglycan layer, so you would probably end up over decolorizing. This would cause a lot of false gram negatives. Probably not. The CVI complex formed between the iodine and crystal violet will be trapped in the lipid layer and not the peptidoglycan layer, so you would probably end up over decolorizing. This would cause a lot of false gram negatives.
Wiki User
∙ 15y ago
Wiki User
∙ 11y agoNo. Iodine acts as the mordant, or the agent used to drive the stain into the bacterial cells. If you use the iodine first, it will take up all the space inside the cells, therefore rendering the stain useless. The stain would have nowhere to go.
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Which stain you should use to stain onion peel?
Both Iodine and Methylene Blue will work - iodine is probably used more often for onion.
What is the function of iodine solution in the gram stain?
It allows the primary stain crystal violet to remain in the cell instead of being washed out. Due to the larger size of the crystal violet molecule, when the ethanol is applied (the decolorizer) the stain will not be washed out of the Gram (+) positive cells.
If leucoplasts stain purple in the presence of iodine what do they store?
starches
Why is it essential that the primary stain and the counterstain be of contrasting colors?
It is essential that primary stain and the counterstain be of contrasting colors so that the target of the primary stain can easily be differentiated on a contrasting background.
Stain name used for onion cells?
safranine stain is used to stain onion cells
What is the importance of iodine after primary stain?
Iodine work as a modrant which helps in fixing the stain properly by making a compled compoun of stain and cell wall.
What is the action of iodine in grams stain?
Iodine is used to bind the Crystal Violet to the Gram Positive microbes.
What is the secondary stain in gram stain procedure?
It is crystal violet & stains all cells purple.
What is the mordant in a gram stain?
Gram's iodine stain is applied after the culture is stained with the primary stain. It acts as a mordant, fixing the primary stain to the cell wall while lending no additional colour to the cell (i.e. the mordant itself is not a stain). The mordant is only able to fix the stain to Gram-positive bacteria because of the characteristic thick, peptidoglycan coat that they possess. Because the mordant is not able to fix the stain to Gram-negative bacteria (who's coat have a different composition), the crystal violet stain will wash away from Gram-negative bacteria when the decolourizing agent is added.
Why gram's iodine called a mordant?
Gram's iodine is called a mordant because it fixes the primary stain to the cell wall and adds any color.
Why is gram's iodine called mordant?
Gram's iodine is called a mordant because it fixes the primary stain to the cell wall and adds any color.
What structure does iodine stain?
iodine
How do you remove iodine stain from paper?
The iodine stain can be removed from the paper by washing the stain in plenty of cold water. Paper distillation can also be used to remove the iodine stain from the paper.
The name given to a liquid used to make cells structures easier to see?
Stain . Iodine is one.
Why do you use iodine to stain a cell?
Iodine stains starch contained in cells. Iodine is also used to distinguish between Gram negative and Gram positive bacteria. The Gram stain contains iodine.
What does lugol's iodine stain do to the activity of the cell?
I just did a lab in biology called "How Plant and Animal Cells Differ" and in the packet it had that question. The answer is: Lugol's iodine stain stops the activity of the cell. It kills whatever specimen it is staining.
What is the difference between Iodine bought in a pharmacy and the Iodine stain solution?
The iodine stain solution you're referring to might be Lugol's iodine. This is iodine and potassium iodide in water. The product available in a pharmacy is tincture of iodine which is iodine and potassium iodide in ethanol and water. Please see the links.
