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What color will gram positive cells become if you fail to use iodine?
Gram positive cells will appear pink if iodine is not used in the Gram staining process. This is because without iodine, the crystal violet stain is not retained by the thick peptidoglycan layer of the cell wall in Gram positive bacteria.
Why use iodine when preparing slide for viewing?
one disadvantage of using iodine to stain cells is that iodine will kill living cells.
What happens to the starch granules of the potato cells when Lugol iodine solution was added?
When Lugol iodine solution is added to potato cells, the starch granules present in the cells appear dark blue or black due to the formation of a starch-iodine complex. This color change is used as a test to detect the presence of starch in cells.
Why onion cells were stained with iodine but rhubarb cells were not?
Onion cells were stained with iodine because they contain starch, which reacts with iodine to produce a blue-black color, allowing for better visualization of the cells. In contrast, rhubarb cells do not contain significant amounts of starch, so staining with iodine would not yield a noticeable color change, making it unnecessary for observation. Thus, iodine staining is used selectively based on the presence of starch in the cells being examined.
Do onion cells still have chloroplasts when they have iodine on them?
The iodine has no connection as to whether the cells have chloroplasts or not. If the cells are green, then they do have them. Adding iodine doesn't change that fact. Not all cells of the plant have chloroplasts. The ones in the soil don't so if you are working with the bulb, it doesn't have chloroplasts in the first place.
After iodine is added to the slide what color do cells appear?
Cells will appear blue or purple after iodine is added to the slide. The iodine reacts with starch in the cells, resulting in a color change that helps to visualize the cellular structures.
List the steps of the gram-staining procedure in order and fill in the color of gram-positive cells and gram-negtive cells after each step?
____________Color of Gram-positive_______Color of Gram-negativePrimary stainCrystal violet_____Purple____________________ PurpleMordant:Iodine____________Purple________________________PurpleDecolorizing agent:Alcohol-acetone__ Purple____________________ ColorlessCounterstain:Safranin___________Purple_________________________Red
When iodine solution is dropped onto plant cells why do dark blue spots appear?
The dark blue spots appear when iodine solution is dropped onto plant cells because iodine reacts with starch in the cells to form a blue-black complex. Starch, which is a polysaccharide made up of glucose units, is stored in plant cells as a source of energy. The dark blue color indicates the presence of starch in the cells.
Why do you use iodine to stain a cell?
Iodine is used to stain cells because it binds to glycogen and starch, making them more visible under a microscope. This helps to visualize the cellular structures and identify specific components within the cell.
What color will gram positive cells become if you fail to use iodine?
Gram positive cells will appear pink if iodine is not used in the Gram staining process. This is because without iodine, the crystal violet stain is not retained by the thick peptidoglycan layer of the cell wall in Gram positive bacteria.
Why use iodine when preparing slide for viewing?
one disadvantage of using iodine to stain cells is that iodine will kill living cells.
How did iodine solution affects the image of the onion cells?
Iodine solution stains the starch granules in the onion cells, making them appear darker under a microscope. This helps visualize the structure and distribution of the starch granules within the cells, providing information about the cellular organization of the onion tissue.
Why is methylene blue is an appropriate dye for staining bacterial cells?
Methylene blue is an appropriate dye because it readily binds to the negatively charged components of bacterial cells, such as nucleic acids and proteins. This binding allows for clear visualization of bacterial morphology and intracellular structures under a microscope. Additionally, methylene blue is a relatively simple and cost-effective stain to use in microbiology.
What happens to the starch granules of the potato cells when Lugol iodine solution was added?
When Lugol iodine solution is added to potato cells, the starch granules present in the cells appear dark blue or black due to the formation of a starch-iodine complex. This color change is used as a test to detect the presence of starch in cells.
What is the radioactive iodine called used to destroy thyroid cells?
The thyroid processes iodine.
Do onion cells still have chloroplasts when they have iodine on them?
The iodine has no connection as to whether the cells have chloroplasts or not. If the cells are green, then they do have them. Adding iodine doesn't change that fact. Not all cells of the plant have chloroplasts. The ones in the soil don't so if you are working with the bulb, it doesn't have chloroplasts in the first place.
What would happen to cells tested with iodine solution?
Cells that contain starch will turn blue-black when tested with iodine solution, indicating the presence of starch. Cells without starch will not change color in the presence of iodine solution. This test is commonly used to identify the presence of starch in cells.
