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Why is methylene blue is an appropriate dye for staining bacterial cells?
Wiki User
∙ 14y ago
It is used in gram staining to differentiate gram negative and gram positive bacteria. After being dyed, the cells are washed with ethanol. Gram positive bacteria will retain the methylene blue due to the amount of peptidoglycan in their cell walls, where gram negative cells will not. Iodine is used as a counter stain, which is up-taken by gram negative cells.
After the gram staining procedure is finished, gram positive cells will appear dark purple or blue due to the retained methylene blue. Gram negative cells will appear pink or red due to the iodine counter stain.
Wiki User
∙ 14y ago
Wiki User
∙ 13y agoIt adheres to the components of the cell structures, so, they are visible under a scope.
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Is endospore staining differencial staining?
Endospore staining is a differential stain used to detect the presence and location of spores in bacterial cells.
Why cant methylene blue be used in place of nigrosin in negative staining?
Because negative staining requires the use of an acidic stain, which will not penetrate the cells because of the negative charge on the surface of the bacteria. As a result, the unstained cells can be easily identified against the colored background.
What bacterial staining technique is it when a basic dye is utilized to stain bacterial cells?
A basic dye used in gram staining is crystal violet.
Why methylene blue can act on only dead yeast cells but not on live yeast cells?
Active enzymes within living cells cause methylene blue to become colorless. Since dead yeast cells have inactive/denatured enzymes, the methylene blue stays blue.
What is the purpose of bacterial staining?
1. It is rapid method of staining 2. useful in case of sterile samples like CSF pleural fluid etc where no. of pus cells and bacteria will be less
What is thee purpose of adding methylene blue to the specimen?
Adding methylene blue to a slide will stain animal cells and make the nuclei more visible.
Staining preparation with eosin and methylene blue?
Eosin is a red stand and methylene blue is blue. The result of staining a bacterial smear with a mixture of eosin and methylene blue is that eosin is acidic and acts as a negative stain. Methylene blue is basic the smear background would turn out red while the cells would turn out blue.
Can food coloring be used to substitute methylene blue in staining cheek cells?
iodine
Why safranine and not methylene blue is used to stain plant cells?
Both are used in staining but for different purposes .
What is the stain blue used to stain animal cells?
Methylene blue is used for many different staining purposes, but one of the main ones is staining RNA or DNA. In animal cells, it will stain the cytoplasm and the nucleus (the nucleus will be much darker).
Is endospore staining differencial staining?
Endospore staining is a differential stain used to detect the presence and location of spores in bacterial cells.
Bacterial smears are fixed before staining to?
affix the cells to the slide and to kill bacteria
Why cant methylene blue be used in place of nigrosin in negative staining?
Because negative staining requires the use of an acidic stain, which will not penetrate the cells because of the negative charge on the surface of the bacteria. As a result, the unstained cells can be easily identified against the colored background.
What bacterial staining technique is it when a basic dye is utilized to stain bacterial cells?
A basic dye used in gram staining is crystal violet.
What is the influence of the pH on the gram reaction in gram staining?
In what regard? You need heat in order to heat fix the bacterial cells to the slide. This adheres cells to the slide. Otherwise, the bacterial cells would wash off the slide during the Gram staining process. If you leave the slide in the Bunsen burner too long, then you can distort the bacterial cell shape and size and also have other artifacts appear on the slide that are not bacterial cells.
Can you heat fix blood smear before staining for bacterial examination?
You absolutely do not heat fix a blood smear before staining, that is, if you are looking at the blood cells. For bacteria, why wouldn't you culture it first and then heat fix, stain etc. I don't think heat fixing the blood stain would damage the bacterial cells so much as make it hard to differentiate the bacterial cells from the dead, shriveled, ruined blood cells, unless maybe you have like an electron microscope or something.
Why is methylene bule nessasary?
Methylene blue is necessary for one thing. It is what helps transports cells.
