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Gram's iodine is called a mordant because it fixes the primary stain to the cell wall and adds any color.
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What is the mordant in a gram stain?
Gram's iodine stain is applied after the culture is stained with the primary stain. It acts as a mordant, fixing the primary stain to the cell wall while lending no additional colour to the cell (i.e. the mordant itself is not a stain). The mordant is only able to fix the stain to Gram-positive bacteria because of the characteristic thick, peptidoglycan coat that they possess. Because the mordant is not able to fix the stain to Gram-negative bacteria (who's coat have a different composition), the crystal violet stain will wash away from Gram-negative bacteria when the decolourizing agent is added.
Why is gram's iodine called mordant?
Gram's iodine is called a mordant because it fixes the primary stain to the cell wall and adds any color.
Why is Iodine used in Gram staining?
Iodine is used in Gram staining as a mordant, which helps to bind the crystal violet dye to the cell wall of bacteria. This mordant-iodine complex forms larger complexes with the crystal violet dye, making it difficult for the dye to be washed away during the decolorization step. This allows for differentiation of Gram-positive and Gram-negative bacteria based on their ability to retain the crystal violet dye.
How many grams of iodine dissolved in water for 0.02 n iodine?
To calculate the grams of iodine dissolved in water for 0.02 N iodine, you need the molar mass of iodine, which is approximately 254 g/mol. With this information, you can use the formula: Grams = Normality (N) * Equivalent weight. Therefore, for 0.02 N iodine: Grams = 0.02 * 254 = 5.08 grams of iodine.
What is iodine number?
Iodine number is a measure of the relative unsaturation of a substance, typically a fat or oil. It indicates the amount of iodine in grams that can be absorbed by 100 grams of the substance, with higher numbers indicating a higher degree of unsaturation. This value is useful in determining the quality and stability of fats and oils for various industrial and nutritional applications.
Given that zinc reacts with iodine in the fixed mass ratio of 1 gram of zinc to 3.88 grams of iodine how many grams of iodine are required to completely react with 4.2 grams of zinc?
For every 1 gram of zinc, 3.88 grams of iodine are required to react. So in this case, with 4.2 grams of zinc, the amount of iodine needed would be (4.2 grams zinc) * (3.88 grams iodine / 1 gram zinc) = 16.296 grams of iodine.
How many moles are in 222.6 grams of iodine?
222,6 grams of iodine is equivalent to 1,754 moles.
What is the function of tincture iodine in trichrome stain?
Tincture iodine in trichrome stain is used as a mordant to enhance the staining of collagen fibers. It helps to improve the contrast and visibility of collagen in the tissue sample.
What mordant is used in the process of gram staining?
The mordant used in the process of gram staining is called crystal violet.
What is the mass of 10 moles of iodine atoms?
Iodine has a molar mass of 129.9g So one mol of Iodine is 129.9g. Therefore 10 moles of iodine would be 1299g.
What is the mordant reagent for?
Mordant reagents are used in staining techniques to help bind dyes to specific structures. Different mordants are needed for different types of dyes and tissue components. For example, in Gram staining, the mordant reagent is iodine, which helps bind the crystal violet dye to the bacterial cell wall.
What does 12.69 stand for in iodine value calculation?
It is the grams of iodine in a 0.1moldm^-3 solution of iodine
