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so dat the bacterial sample smear can be mixed with the primary stain throgh heating

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What stains best replace carbol fuschin in gram staining?

In Gram staining, carbol fuchsin can be replaced by other stains such as safranin or crystal violet for the primary stain. Crystal violet is commonly used as it provides a strong initial color to the Gram-positive bacteria. Safranin, typically used as a counterstain, can also serve as an alternative for carbol fuchsin, particularly in modified staining protocols. However, the choice of stain may affect the clarity and contrast of the results.


Does fresh ham butt have an odor?

Fresh ham butt should not have a strong or unpleasant odor. It should have a mild, meaty smell. If it has a strong or foul odor, it may indicate that the meat is no longer fresh and should not be consumed.


What safety label should be on a bottle of strong alkali?

The label is corrosive.


If the weather map shows many pressure gradients close together what things should you take to be prepared for the day's weather?

You should be prepared for strong wind.


Describe the mechanism of action for acid-fast stain?

ACID FAST Os Contains waxlike lipoidal material affecting staining quality. Carbolfuchsin is primary stain. Acid fast organisms resist decolorization with acid alcohol. After decolorization, methyelene blue is added to organisms to counterstain any material that is not acid fast. It is used for examination of Mycobacterium. The principle of staining is depend on the resistance of this type of bacteria to decolarization by acid alcohol, because the cell wall contain waxy material (mycolic acid) which prevent the removal of carbol fuchsin from the cell. Staining Procedure 1. Flood the slide with strong carbol fuchsin and heat until steam rises (but do not boil). 2. After 3-4 min apply more heat until rises again; do not let the stain dry on the slide. 3. About 5-7 min after the first application of heat washes the slide thoroughly under running water. 4. Decolourize in acid-alcohol until all traces of red have disappeared from the film. Decolourization should not be attempted in one stage; there should be intermittent washings in water and re-application of acid-alcohol. 5. Wash well with water and counter stain with Methylene blue for 1 min. 6. Wash and stand end to drain. 7. Acid-fast organisms are red, other organisms are blue.

Related Questions

What stains best replace carbol fuschin in gram staining?

In Gram staining, carbol fuchsin can be replaced by other stains such as safranin or crystal violet for the primary stain. Crystal violet is commonly used as it provides a strong initial color to the Gram-positive bacteria. Safranin, typically used as a counterstain, can also serve as an alternative for carbol fuchsin, particularly in modified staining protocols. However, the choice of stain may affect the clarity and contrast of the results.


How was Hurricane Katrina different from flooding?

The Mexican ocean flooded into inland as waves was high by really Strong wind


What would happen when Singapore is heavily flooded?

When Singapore is heavily flooded by strong rain, I believe there will be some causalities. Shops will be affected but majority of the houses wont since most are high rising. If Singapore is flooded due to rise of sea level, I suppose the number of countries on Earth will be only 180.


Why does your 1987 Toyota SR5 has a strong gas odor when running how can you solve this problem?

Look for a fuel leak Injectors flooding? Carbon canister flooded and need replacing?


How can you know car is A flooded engine?

Removing the spark plugs and finding the spark plugs soaked with gasoline. Also the engine oil may have a strong odor of gasoline.


In a flooded area the water does not have to be deep to sweep you off your feet.?

In a flooded area, even shallow water can pose significant risks due to strong currents and debris. Fast-moving water can easily knock a person off balance, leading to potential injury or drowning. Additionally, the force of the water can be deceptive; what seems manageable can quickly become overwhelming. It's essential to exercise caution in flooded conditions, regardless of the water's depth.


When your ex says he's strong?

He might be strong if he is saying that. He will not remember you if he is that strong. You should get over that as well.


How strong should your hamstring be?

your hamstring should be as strong as your quad so that the force exerteted on the leg will be equal to both muscles


Salvage yards should keep a strong inventory list. ?

Salvage yards should keep a strong inventory list.


What are the properties of flight?

weapon system should be strong. speed should be very good. fuel system should be strong and big.


How do you be strong?

To become strong, you should exercise daily and eat healthy foods.


Why teens should not marry?

they should, because it makes them strong