Eosin methylene blue (EMB) agar is selective due to the presence of dyes eosin Y and methylene blue, which inhibit the growth of Gram-positive bacteria. This allows for the selective isolation of Gram-negative bacteria, particularly coliforms.
Most gram positive bacteria do not grow on MacConkey agar or Eosin Methylene Blue (EMB) agar, as these are selective media designed to inhibit the growth of gram positive organisms. So, typically, you would not expect a gram positive bacteria to grow on these media.
EMB is an undefined selective/differential medium. It contains aniline dyes (methylene blue and eosin), which inhibit the growth of Gram-positive bacteria selecting for Gram-negative bacteria. EMB also contains lactose which makes the media differential based on an organisms ability to ferment lactose
Mycobacterium phlei is a non-fastidious organism that is commonly found in soil and water. It is not commonly grown on Eosin Methylene Blue (EMB) agar, as EMB agar is more selective for gram-negative enteric bacteria. Instead, Mycobacterium phlei is typically cultured on media specifically designed for mycobacteria, such as Middlebrook agar or Lowenstein-Jensen agar.
Eosin and methylene blue dyes present in EMB (Eosin Methylene Blue) agar inhibit the growth of gram-positive organisms by interfering with their ability to take up nutrients and causing damage to their cell walls. This makes the medium selective for gram-negative bacteria, allowing them to grow while inhibiting the growth of gram-positive bacteria.
EMB (Eosin Methylene Blue) agar is a selective and differential medium used for the isolation of fecal coliforms from water samples. The dyes eosin and methylene blue inhibit the growth of Gram-positive bacteria, allowing for the selective growth of Gram-negative bacteria such as E. coli. The formulation also allows for the differentiation of lactose fermenters (pink/purple colonies) from non-lactose fermenters (colorless colonies).
The yeast.
Most gram positive bacteria do not grow on MacConkey agar or Eosin Methylene Blue (EMB) agar, as these are selective media designed to inhibit the growth of gram positive organisms. So, typically, you would not expect a gram positive bacteria to grow on these media.
when you plate E.coli onto a EMB plate the colonies turn shinie metalic green, EMB is selective for gut bacteria and it diferentiates E.coli.
EMB is an undefined selective/differential medium. It contains aniline dyes (methylene blue and eosin), which inhibit the growth of Gram-positive bacteria selecting for Gram-negative bacteria. EMB also contains lactose which makes the media differential based on an organisms ability to ferment lactose
Pseudomonas aeruginosa can grow on Emb agar, as it is a selective and differential growth medium commonly used to isolate and differentiate members of the Enterobacteriaceae family, including P. aeruginosa. Its growth on Emb agar will exhibit the characteristic green metallic sheen due to its ability to produce pyocyanin pigment.
Eosin methylene blue (EMB) agar is selective for gram-negative bacteria, especially those that can ferment lactose. Common bacteria that grow on EMB agar include Escherichia coli and Enterobacter aerogenes. These bacteria are differentiated by their ability to ferment lactose, producing colonies that are metallic green or dark purple on EMB agar.
Mycobacterium phlei is a non-fastidious organism that is commonly found in soil and water. It is not commonly grown on Eosin Methylene Blue (EMB) agar, as EMB agar is more selective for gram-negative enteric bacteria. Instead, Mycobacterium phlei is typically cultured on media specifically designed for mycobacteria, such as Middlebrook agar or Lowenstein-Jensen agar.
So here is the deal, same use different recipies different results to do the same thing. I'll just post some lecture notes and hope that helps:MacConkey's Agar (enterics)Selective for gram negativeCrystal violet and bile salts prevent non-entericsDifferential for lactose fermentationNon-fermenter --> white/clear coloniesFermenter--> red/pink coloniesEosin Methylene Blue (EMB) (Weakly selective for intestinal pathogens)Selective for gram negativeEosin and methylene blue inhibit gram positive growthDifferential for lactose fermentationLactose fermenters produce a green-metallic sheenNon-fermenters do notNOTE: EMB Agar is not in common use anymore
the main ingridient of a vaccination is microbe apon other things
EMB is an undefined selective/differential medium. It contains aniline dyes (methylene blue and eosin), which inhibit the growth of Gram-positive bacteria selecting for Gram-negative bacteria. EMB also contains lactose which makes the media differential based on an organisms ability to ferment lactose.
it is selective because only a salt tolerant can grow. 7.5 nacl it has diffrential properties also. if mannitol formentors turns yellow staphlococus aureus; but if it has no change then its a nonpathogenic staphlococci (s. edermis)
Eosin and methylene blue dyes present in EMB (Eosin Methylene Blue) agar inhibit the growth of gram-positive organisms by interfering with their ability to take up nutrients and causing damage to their cell walls. This makes the medium selective for gram-negative bacteria, allowing them to grow while inhibiting the growth of gram-positive bacteria.