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Which of the availble stain enhance your observation the most methylene blue or iodide?
Methylene blue would enhance your observation more than iodide staining. Methylene blue binds to nucleic acids and proteins, making them more visible under a microscope. Iodide staining is primarily used for visualizing lipids in samples such as plant tissues.
What color is simple staining on a bacillus and coccus using methylene blue stain?
Methylene blue stains everything blue.
What is thee purpose of adding methylene blue to the specimen?
Adding methylene blue to a slide will stain animal cells and make the nuclei more visible.
How would the pH of the stain methylene blue affect the staining of bacteria?
The pH of the methylene blue stain can impact its ability to adhere to bacteria and penetrate the cell wall. A lower pH may enhance staining by increasing the positive charge of the dye, allowing it to bind more effectively to the negatively charged bacterial cell wall components. Conversely, a higher pH could reduce the staining efficiency by decreasing the positive charge of the dye.
What is the substance that have the same function as methylene blue?
One substance that has a similar function as methylene blue is crystal violet. It is commonly used in staining techniques for microbiological studies and exhibits similar properties in terms of staining cells and tissues.
Can food coloring be used to substitute methylene blue in staining cheek cells?
iodine
Staining preparation with eosin and methylene blue?
Eosin is a red stand and methylene blue is blue. The result of staining a bacterial smear with a mixture of eosin and methylene blue is that eosin is acidic and acts as a negative stain. Methylene blue is basic the smear background would turn out red while the cells would turn out blue.
Can dyes or other methylene blue be used for direct staining?
Crystal violet, basic fuchsin, and safranin are all dyes which can be used in direct staining because they are cationic which means that they are positively charged. These dyes which are positively charged will react to the bacterial cell wall because the cell wall is negatively charged resulting in a basic stain.
What color is methylene blue?
Methylene blue is a synthetic dye that appears as a dark blue or greenish-blue color in its solid form. It is commonly used in biological staining techniques and as a medication for certain medical conditions.
Is methylene blue a chromophore?
Nope - methylene blue is a stain. You need liquid indicator like phenolphthalein that responds to pH changes.
Why safranine and not methylene blue is used to stain plant cells?
Both are used in staining but for different purposes .
What is methylene blue use for when gram staining?
Methylene blue can be used to prepare slide for animal cells. For example, if you want to examine a piece of your cheek cell, you would take a piece of cotton wool and rub it on the inside of your cheek and the rub it on a microscope slide, the you add a little distilled water and then a drop of methylene blue. The final step you would take, is to cover the slide with a cover slip, then place it under the microscope.
