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Describe the microscopic appearance of encapsulated streptococcus if stained with safranin?

Safranin (red) is used in gram staining and endospore staining as the secondary stain. Nigrosin is used in negative staining, staining only the background and not the bacteria. Therefore, the bacteria within the capsule would stain red from the safranin. (Like in endospore staining and negative gram staining, safranin would stain the bacteria red.) Nigrosin would stain the background of the organism just as it would in negative staining. Bacteria (within capsul): stained safranin red Capsule (outer layer of bacteria): clear Background of organism: stained dark with Nigrosin


If one stained a gram-positive cell only with safranin?

If a gram-positive cell is stained only with safranin, it would likely appear pink or red under a microscope. This is because safranin is a counterstain used in the Gram staining procedure to colorize gram-negative bacteria, whereas gram-positive bacteria retain the crystal violet primary stain and appear purple.


What would you expect to see inside the stained cells nucleus?

It would be the color you stained.


Why is counterstaining necessary when using a differential staning technique such as the gram stain?

The counter-stain allows you to see all the structures that were not stained with the primary stain. Without the counter-stain, all you would see is the purple-stained structures (nucleus, some cytoplasmic proteins), but you would have a difficult time observing the cell membrane and many cytoplasmic structures.


Who manufactured a lipstick with color named stained glass?

It was made by Fabergé in the 1960s. Wish they would resurrect it.


What happens if not enough alcohol is applied to a gram stain?

Both bacteria types would be stained by the safranin. When the iodine is added, safranin would be "set" in the positive. The decolorizer would wash out the safranin and then application of the crystal violet would stain the negative.


What would happen if you forgot to counterstain with safranin?

Gram neg cells would remain clear


If Congo red is used instead of safranin in the gram stain technique what would happen?

Using Congo red instead of safranin in the Gram stain technique would not provide accurate results. Safranin is essential for counterstaining gram-negative bacteria, whereas Congo red would not differentiate between gram-positive and gram-negative cells due to its staining properties. This would lead to incorrect classification of bacteria in the Gram stain.


What color stain would you prefer for an oak executive desk?

Oak is very versatile and so can be stained many colors. For an oak executive desk I recommend a natural color stain or a golden stain. Both would be very attractive.


What would be the appearance of the gram positive bacterium if you forget to counterstain with safranin?

If you forget to counter stain color of Gram positive would be violet or blue . The above answer is good. Here is why the above answer is good. Yes it would still be Violet or blue. Gram positive bacteria are gram positive, because it holds onto the crystal violet stain that washes out of gram negative bacteria. Counterstaining with safranian turns gram negative bacteria pink to red only because the crystal violet has washed out of the gram negative. The lighter safranian has little to no effect on gram positive bacteria. The cause of the difference has to do with the makeup of the cell wall in the different bacteria.


What colour would you expect the mycoplasma to stain with gram reagents?

Mycoplasma does not have a cell wall, so it does not stain with traditional Gram reagents like crystal violet and safranin. Instead, it is commonly stained using specialized stains like DAPI (4',6-diamidino-2-phenylindole) for visualization under fluorescence microscopy.


What color is a rough ER?

I cannot tell you specifically what color the Endoplasmic reticulum is, because it varies plant to plant and animal to animal. But thus, with slight variation, it appears to be a pinkish colour