Vulture was going to be the bad guy in "Spider-Man 4", but the film was cancelled and now the series is being rebooted. So the Lizard is going to be the bad guy in "The Amazing Spider-Man" which will be the first time Dr. Curt Connors transforms into his villainous alter ego.
if you happen to be talking about the second symbiote in spider man, then it varies greatly on the game you are playing. ANY of the movie games, I don't think you can. For the original spider man, no. I don't know about any of the spider man friend or foe or other games like that.