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That kind of depends on what you mean by substance. An electron is a piece of matter that isn't made of chemicals. Quarks aren't made of chemicals. Neither are neutrinos, or photons, or Z bosons, or any other subatomic particles; strange exotic materials like neutronium or quark-gluon plasmas also aren't made of chemicals. However, any ordinary everyday substance big enough to see or feel or smell is made of chemicals.

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Q: Is there any substance not made of chemicals?
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