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What is inner membrane protein and outer membrane protein How can you get membrane protein from E. coli and how to separate the inner and outer membrane protein?
Inner membrane proteins are embedded in the inner membrane of bacteria, while outer membrane proteins are located in the outer membrane. To obtain membrane proteins from E. coli, you can disrupt the cells through methods like sonication or French press, followed by using differential centrifugation to separate the inner and outer membrane fractions based on their densities. Further purification techniques such as density gradient ultracentrifugation or membrane solubilization can be used to isolate inner and outer membrane proteins specifically.
A researcher made an interesting observation about a protein used to build a cell's plasma membrane The final protein in the plasma membrane was slightly different from the protein made by the ER Th?
Golgi Apparatus
Who developed a telegraph that used electromagnetisims to send and receive signals?
I did!!
Who developed a telegraph that used electromagnetism to send and receive signals?
Samuel Morse
Are proteins found in the cell membrane?
There are many uses for proteins in a cell membrane but the most common ones are: 1. Facilitated diffusion- a protein acts almost like a channel to allow molecules that cannot fit across the phospholipid bilayer to diffuse into/out of the cell 2. Active transport- proteins are used to move particles across the membrane from low to high concentration, using energy. 3. As receptor molecules which take signals from things like hormones.
What is the difference between regular protein and g protein?
Regular protein is used by the body for nutrition. G protein is used by the body for transmitting signals and messages between various cells. More detailed information is available on Wikipedia.
A researcher made an interesting observation about a protein used to build a cell's plasma membrane The final protein in the plasma membrane was slightly different from the protein made by the ER?
The observed difference between the final plasma membrane protein and the ER protein could be attributed to post-translational modifications. These modifications, such as glycosylation or phosphorylation, can alter the protein's structure and function during its journey through the cell to its final destination in the plasma membrane. This dynamic process allows cells to fine-tune protein function for specific roles in different cellular compartments.
Energy used to establish the protein gradient across the thylakoid membrane comes from where?
Synthesis of ATP
What form of transport is used by the cell membrane to speed up the intake of glucose?
Facilitated diffusion is the transport process used by the cell membrane to speed up the intake of glucose. This process involves the use of protein channels or carriers to help glucose molecules pass through the membrane.
What a sateliite dish is for?
A Satellite Dish is used to receive and transmit signals which contains different information to or from the satellite.
In cells of multicellular organisms the cell membrane contains molecules involved in cell communication what specific term is used to identify these molecules?
That kind of membrane in cells is called a mosaic membrane since the molecules are not all the same.
Why you use skim milk to block the membrane in western blotting?
The membrane binds proteins unspecifically (it will bind any).Western blotting uses antibodies to probe the proteins bound to the membrane for the one you are testing for. The antibodies bind specifically to a particular protein that you have chosen and contain a label to allow identification of them on the membrane. In this way you can see whether or not the membrane, and therefore the tissue you are testing, contains a particular protein.Antibodies are proteins. This means the antibody would bind anywhere on the membrane, as well as on the protein you're looking for, rendering the results worthless (it will ALWAYS show positive). To stop the antibodies binding to the membrane, so that they only bind to the protein (if it exists) the membrane must be blocked (completely filled with protein) so that there is nowhere on the membrane for the antibody to bind.Since milk contains a generic mix of a relatively high concentration of known proteins, it is used to block the parts of the membrane that don't already have protein on so that the antibodies won't bind there. As it is very cheap and readily available, it is a good source of protein to use. It is unlikely that a Western blot would be needed for any protein that is contained in milk, but if that is the case an alternative mixture can be used.
