Yes.
Sometime allies Carthage and Rome took sides in internal Sicilian wars and so became enemies.
The Mamertines, mercenaries from Campania near Naples, seized the city of Messana (todays's Messina) eastern Sicily, on the strait which separates the island form the mainland. They killed all the men and took the women as their wives.
Hierro II of Syracuse defeated the Mamertines in battle. The Mamertines asked both Carthage (which had ports in western Sicily) and Rome for help. Carthage agreed to help but wanted to set up a Carthaginian garrison in Messana. The Marmetines asked Rome for help again. The senate was reluctant to wage war. However, the assembly of the soldiers voted for war.
There were three wars. The first began as a dispute over Sicily between Carthage and Rome.
First Punic War: Carthage was pushing its control in Sicily and ran up against some rebellious mercenary soldiers. Rome chose to support them as a means of having an excuse to move into Sicily to expand its territory beyond Italy. Second Punic War: Carthage attacked Sargentum in Spain, in breach of the Ebro River line agreed with Rome as dividing their areas of interest in Spain.
They were trying to expand into Sicily and confronted the Carthaginian moves there, pretending to assist the locals, who were really rebellious Roman mercenaries.
Rome intervened in Sicily to help mercenaries (the Mamertines) who had seized the city of Messana, (Messina, in the northeast, on the strait which separates the island from the mainland) who were attacked by Syracuse, the most powerful of the Greek city-states in the east and south of the island. The Carthaginians worried about the safety of their four ports in western Sicily and mobilised for war with Rome.
The Roman Republic and the Carthaginian Empire were looking for land, when there army's collided and fought to the death for the land Sicily.
There is a timeline of events regarding the Punic Wars at the related link below .
Before the wars Carthage controlled much of Northern Africa, southern Spain and had a foot hold in Sicily. They also had dominance over the seas and until the Punic wars had an unmatched and vast trade fleets. Much of Carthage's wealth came from control of trade roots.
264 BCE.
The Second Punic War stated in 218 BC and ended in 202 BC.
In 264 BC.
218 BCE.
The Punic Wars started in 264 BC as a result of territorial disputes between Rome and Carthage.