A low temperature can slow down enzyme activity and high temperatures can denature an enzyme making it unusable. pH levels also affect enzyme activity. Every cell has an ideal temperature and pH
There are 4 factors. Temperature, pH, enzyme concentration, and substrate concentration.
Temperature increases enzyme activity till an optimum temperature which after that drastically reduces its activity due to the enzyme protein denaturing.
All enzymes function at a specific pH range, (change in pH results in change in structure due to the ionic bonds present between amino acids)
high enzyme concentration results in higher effective collision between enzyme and substrate. therefore activity increases.
High substrate concentration results in higher activity as the rate of effective collision increases, but after a certain concentration the rate would be constant as all enzyme active sites are maxed out.
The temperature, the pH value, the substrate concentration and the enzymes concentration influence enzyme activity.
Temperature plays an important role in enzyme activity. Temperature increases the reaction of enzymes, however it can only reach a certain temperature. If the temperature goes above 37C, the enzyme will denature.
Because they are catalysts for reactions, enzymes can be affected by any variable that influences a chemical reaction. Many enzymes are affected by changes in temperature.
Concentration ( enzyme to substrate ), temperature and pH.
Temperature or pH.
Enzymes are catalysts that speed up chemical reactions. Some environmental factors that can affect enzyme activity are temperature and pH levels.
Blocking of enzyme activity depends on various factors such as concentration of substrates, pH, temperature, some durgs. As for example rifampicin durg blocks RNA polymerase II activity.
Non-protein compounds called co-factors are required for some proteins in order to facilitate enzymatic activity. Common examples of co-factors are metal ions (Ca2+, Mg2+ etc.), organic co-factors such as heme (in hemoglobin), among others.
** TEMPERATURE ** pH LEVEL ** PARTICLE SIZE ** SUBSTRATE CONCENTRATION
BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. This protein does not affect other enzymes that do not need it for stabilization.
Enzymes are catalysts that speed up chemical reactions. Some environmental factors that can affect enzyme activity are temperature and pH levels.
Blocking of enzyme activity depends on various factors such as concentration of substrates, pH, temperature, some durgs. As for example rifampicin durg blocks RNA polymerase II activity.
Factors affecting enzyme activity1: Enzyme concentrationIf the quantity of enzyme is doubled, the enzymatic activity will also be doubled because more enzymes are now available to work. After a certain level of enzyme concentration, there will be no more increase in the enzymatic activity because all the substrate molecules are combined with an enzyme and the rate of reaction will stabilize.2: Substrate concentrationBy increasing substrate concentration, enzymatic activity increases. Increasing the substrate further without increasing the enzyme concentration will not affect the enzymatic activity because all the enzymes are occupied by a substrate molecule.3: pH valueSome enzymes require acidic surroundings, most require a more neutral condition for their activity. Change in the pH can change the enzyme's structure and enzyme become useless.4: TemperatureAn increase in temperature of 10 degree celsius doubles the enzymatic activity. Each enzyme has its own optimum temperature at which its enzymatic activity is maximum. Very high temperatures break the bonds that maintain shape of enzyme. If the enzyme denatures, the substrate can not fit in to the active sites and enzyme become useless.
This depends on the enzyme. Temperature, pH, and the presence of co-factors, or allosteric inhibitors.
Non-protein compounds called co-factors are required for some proteins in order to facilitate enzymatic activity. Common examples of co-factors are metal ions (Ca2+, Mg2+ etc.), organic co-factors such as heme (in hemoglobin), among others.
have a shape that fits into the enzyme
One is cofactor A.
Temperature, rainfall, vegetation, animal activity, type of rock being weathered.
Substance concentration, enzyme concentration, temperature and PH level
At a high ion concentration, the ion interfere with the bonds between the side groups of the amino acids making up the enzyme (which is a protein). This causes the enzyme to lose its shape, called denaturation. If the enzyme loses its shape, it can no longer accept and react substrate, so the rate of enzyme activity decreases.
Hydrogen peroxide (H2O2) is a poisonous byproduct of metabolism that can damage cells if it is not removed. Catalase is an enzyme that speeds up the breakdown of hydrogen peroxide into water (H2O) and oxygen gas (O2).
Locamotion, cell movement, biosynthesis