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What is the function of restriction enzymes that naturally occur in bacteria cells?
Wiki User
∙ 13y ago
Restiriction enzymes, or endonucleases, splice (cut) apart two different sites of the nucleotide sequence on foreign DNA resulting in two different pieces of DNA for a gene of interest to be inserted. This usually occurs in bacteria such as E. coli and such. Once the two cuts have been made (one at 5' and one at 3') there remains the open ends of the DNA called 'sticky ends'. THis is where the gene of interest is inserted.
Wiki User
∙ 13y ago
Wiki User
∙ 13y agorestriction enzymes are the enzymes that can cut the DNA at particular sequences ( endonuclease ) . the basic function in bacteria is to protect from phage (viral) DNA i.e., when a bacteriophage attacks the bacteria it sends its genetic material into the bacterial cell , this genetic material then uses the host machinery to produce the viral copies , in order to prevent it ,the bacteria uses the restriction enzyme to cleave the genetic material of the virus and hence renders it useless .
Wiki User
∙ 9y agoThe natural use of restriction enzymes by bacteria is to destroy viral DNA. Enzymes used to cut genes in recombinant DNA research are restriction enzymes.
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Why do bacteria naturally contain restriction enzymes?
Restriction enzymes are the bacteria's form of an 'immune system' against viruses (which can infect bacteria). When viruses try to insert their own DNA into a bacteria's genome, the restriction enzymes detect this foreign DNA and cut it out so that the viruses can't replicate and kill the cell.
Bacteria methylate their DNA in order to?
Protect their DNA from being excised by their own restriction enzymes such as EcoR1. The bacteria has enzymes that go through and methyl the N6 position of their adenines in order to prevent their own restriction enzymes from cutting their DNA up.
Which pair of enzymes is necessary to make recombinant DNA?
Actually the answer would be Restriction enzyme and DNA ligase.
What is the role of restriction enzymes in bacteria?
Restriction enzyme in bacteria cuts of the foreign DNA inside the host, thus destroying them. The host DNA is protected against this destructive action due to methylation of the host DNA.
Enzymes used to cut DNA molecules in recombinant DNA researsh are?
restriction enzymes
Are bacteria a source of restriction enzymes in DNA?
Restriction enzymes are made by the ribosomes in bacteria. For information on which bacteria produce which specific restriction enzymes, some can be found in a table near the bottom of this page: http://en.wikipedia.org/wiki/Restriction_enzymes
Why do bacteria naturally contain restriction enzymes?
Restriction enzymes are the bacteria's form of an 'immune system' against viruses (which can infect bacteria). When viruses try to insert their own DNA into a bacteria's genome, the restriction enzymes detect this foreign DNA and cut it out so that the viruses can't replicate and kill the cell.
why do bacteria have restriction enzymes?
to destroy viral DNA
What is the enzyme function of restriction enzymes?
We believe the function of the restriction enzyme is to protect an organism from foreign DNA as restriction enzymes cleave DNA strands (making them useless). The idea is that bacteria use this to protect against viral infection as viruses attach to the cell and insert their DNA into it in order to "take over" the cell. Restriction enzymes recognize this DNA as foreign and begin to chop it up, saving the cell.
How have scientists made bacteria more useful today?
Scientists have taken restriction enzymes out of bacteria; restriction enzymes are used to cut DNA at cut sites. Also, they insert genes into bacteria to study them.
What does a geneticist use to cut DNA at specific base sequences?
Restriction enzymes, also known as restriction endonucleases, are used to cut DNA into smaller fragments. Restriction enzymes are found in bacteria, where they act like molecular scissors by cutting up DNA from invading viruses or bacteriophages. Each restriction enzyme recognizes a specific nucleotide sequence and cuts the DNA at that site. This process makes restriction enzymes extremely useful in biotechnology where they are used in procedures such as DNA cloning, DNA fingerprinting, and genetic engineering. There are hundreds of known restriction enzymes, and each one was named for the bacteria from which it was isolated. For example, EcoRI was isolated from Escherichia coli and HaeIII from Haemophilus aegyptius.
Bacteria methylate their DNA in order to?
Protect their DNA from being excised by their own restriction enzymes such as EcoR1. The bacteria has enzymes that go through and methyl the N6 position of their adenines in order to prevent their own restriction enzymes from cutting their DNA up.
What does restriction endonuclease do?
They cut DNA at specific sequences. Restriction endonucleases work by cutting DNA at specific sequences. The places that are cut are known as restriction sites.
Where restriction enzymes come from?
Restriction enzyme, also called restriction endonuclease, a protein produced by bacteria that cleaves DNA at specific sites along the molecule. In the bacterial cell, restriction enzymes cleave foreign DNA, thus eliminating infecting organisms.
What enzyme do scientists use to cut genes out of strands if DNA?
restriction enzymes
What organisms make restriction enzymes?
Originally restriction enzymes (RE) are isolated from Bacteria and Archaea. Microorganisms uses REs to protect themselves from the viral infection. For example EcoRI is isolated from E.colianbd BamHI is found in Bacillus amyloliquefaciens. With the advent of rDNA technology these enzymes are now being produced in lab organisms by cloning and expression.
Can virus get a bacteria?
Yes- Bacterial viruses are called bacteriophages. Some examples are T4 and T7 phages. They are complex DNA viruses that attach themselves to the cell surface and then inject their DNA to the inside of the bacteria. Bacteria have enzymes called restriction enzymes that cleave DNA at specific sites called restriction sites. These enzymes are the bacterium's defense against phages.
