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Yes, you can start a sentence with "again" as it functions as an adverb to indicate repetition or resumption of an action, such as "Again, we discussed the project timeline during the meeting."
In your case, the question is, can a sentence come to a conclusion. Try again.
It means that that you have to start all over again. Imagain a game of snakes and ladders, you fail, so you go down a snake to square. You have to do it all again. back to square 1
It really depends upon the sentence. Remember, a comma indicates a slight pause. If you would pause when speaking that sentence at that location, then put in a comma to indicate that.
Well, to put it in a nutshell, we're going to have to start again.
First, you have to be a resident of Connecticut. After that, you have to start from square one again.
No, you cannot start a sentence with "no" unless you need to do so. No reason exists not to start a sentence with that word, as you can see. No one will care if you do start a sentence with it.
Yes, I can start a sentence with "Is."
Yes, you can start a sentence with a verb.
Yes, you can start a sentence with an acronym.
You can start a sentence with "Also"
You start a sentence with whatever word you need to start it with. A sentence can start with "A" if it needs to. A sentence just needs to make sense.