What is spatial transcriptiomics?

Answer 1

The fundamental idea is to retain spatial information while profiling gene expression in the tissue. Thin sections of tissue are mounted onto slides that contain an arrangement of spots or arrays, each with unique molecular barcodes or capture probes. After that, the tissue sections on the slides are treated so that mRNA molecules can bind to the capture probes or barcodes on the array. Since each capture probe is designed to target a specific gene or set of genes, it becomes possible to identify RNA molecules that correspond to the expressed genes by applying reverse transcription and DNA sequencing. Lasst but not least, the spatial information from each array can be combined with the results of the gene sequencing, resulting in a spatial gene expression map of the tissue.

To learn more about about human tissue samples and how they are used, I recommend you to visit our website, where we gathered more information and some FAQs:

centralbiohub.de/biospecimens/tissue-bank/human-tissue-samples